IGEM:IMPERIAL/2006/project/Bio elec interface
From OpenWetWare
<html><style type='text/css'> .tabs {
font-size:100%; font-weight:none; width: 100%; color:white; background:white;
}
.tabs li {
background:white;
}
.tabs a,.tabs strong {
background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top; color:white; padding: 3px 10px 3px 4px;
}
.tabs strong{
color:#F20D0D; background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
}
.tabs a:hover{
color:red;
}
</style></html>
Biological to Electrical Interface
Specifications
Bio Reporter | Signal Transducer |
---|---|
Expressed by E.coli | Detect Bio reporter |
Degradation in medium afterwards. Time needed for degradation should be equal to/less than that of GFP | Specific response to bio reporter only |
No other interaction with E.coli processes | Real-time readout |
Sufficient expression and secretion of bio reporter in medium for detection by signal transducer | Sufficient sensitivity to detect changes in bio reporter |
Commercially available |
Possible Implementation
- Signal transduction could involve (protein) redox-reactions
- Antigen/Antibody sensors?
- Protein sensors
- Hormone/Neurotransmitter sensors (e.g. serotonin)
- Scenario 1:
- E.coli is in a liquid medium
- Some bioprocess triggers production of a bio reporter (protein) in E.coli
- The bio reporter is expelled from the cell into the medium
- A sensor will measure the level of bio reporter (protein) in the medium
- Scenario 2:
- E.coli is in a liquid medium
- Some bioprocess triggers productio of a bioreporter (protein) in E.coli
- The bio reporter is expelled from the cell into the medium
- There, the bio reporter (protein/enzyme) breaks down a certain chemical present in the medium
- A commercially available sensor then measures the
- a) increase in concentration of the breakdown products or,
- b) the decrease in concentratio of the initial chemical