IGEM:IMPERIAL/2006/project/Bio elec interface
From OpenWetWare
Biological to Electrical Interface
Specifications
Bio Reporter | Signal Transducer |
---|---|
Expressed by E.coli | Detect Bio reporter |
Degradation in medium afterwards. Time needed for degradation should be equal to/less than that of GFP | Specific response to bio reporter only |
No other interaction with E.coli processes | Real-time readout |
Sufficient expression and secretion of bio reporter in medium for detection by signal transducer | Sufficient sensitivity to detect changes in bio reporter |
Commercially available |
Possible Implementation
- Signal transduction could involve (protein) redox-reactions
- Antigen/Antibody sensors?
- Protein sensors
- Hormone/Neurotransmitter sensors (e.g. serotonin)
- Scenario 1:
- E.coli is in a liquid medium
- Some bioprocess triggers production of a bio reporter (protein) in E.coli
- The bio reporter is expelled from the cell into the medium
- A sensor will measure the level of bio reporter (protein) in the medium
- Scenario 2:
- E.coli is in a liquid medium
- Some bioprocess triggers productio of a bioreporter (protein) in E.coli
- The bio reporter is expelled from the cell into the medium
- There, the bio reporter (protein/enzyme) breaks down a certain chemical present in the medium
- A commercially available sensor then measures the
- a) increase in concentration of the breakdown products or,
- b) the decrease in concentratio of the initial chemical
Summary of meeting with Professor Tony Cass
Summary of meeting with Dr. Anna Radomska
Further Reading
- pH-based enzyme potentiometric sensors. Part 1. Theory
- pH-based enzyme potentiometric sensors. Part 2. Glucose-sensitive field effect transistor
- pH-based enzyme potentiometric sensors. Part 3. Penicillin-sensitive field effect transistor
- Response of an enzyme-modified pH-sensitive ion selective device; consideration of the influence of the buffering capacity of the analyte solution
- Response of an enzyme-modified pH-sensitive ion-selective device; experimental study of a glucose oxidase-modified ion-sensitive field effect transistor in buffered and unbuffered aqueous solution
- Response of an enzyme-modified pH-sensitive ion-selective device; analytical solution for the response in the presence of pH buffer