IGEM:IMPERIAL/2006/project/Bio elec interface - Revision history

Vincent Rouilly at 16:48, 30 October 2006

Vincent Rouilly — Mon, 30 Oct 2006 16:48:41 GMT

←Older revision		Revision as of 16:48, 30 October 2006
Line 71:		Line 71:
	::a) increase in concentration of the breakdown products or,		::a) increase in concentration of the breakdown products or,
	::b) the decrease in concentratio of the initial chemical		::b) the decrease in concentratio of the initial chemical
		+
		+
		+	<html>
		+	<!-- Start of StatCounter Code -->
		+	<script type="text/javascript" language="javascript">
		+	var sc_project=1999441;
		+	var sc_invisible=1;
		+	var sc_partition=18;
		+	var sc_security="18996820";
		+	</script>
		+
		+	<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&java=0&security=18996820&invisible=1" alt="website statistics" border="0"></a> </noscript>
		+	<!-- End of StatCounter Code -->
		+	</html>

Cys at 09:05, 1 September 2006

Cys — Fri, 01 Sep 2006 09:05:37 GMT

Show changes

Cys at 11:09, 25 August 2006

Cys — Fri, 25 Aug 2006 11:09:18 GMT

←Older revision		Revision as of 11:09, 25 August 2006
Line 1:		Line 1:
-	~~<html><style type='text/css'>~~	+	{{IGEM:IMPERIAL/2006/Biosensor}}
-	~~.tabs~~ {	+
-	~~font-size:100%;~~	+
-	~~font-weight:none;~~	+
-	~~width: 100%;~~	+
-	~~color:white;~~	+
-	~~background:white;~~	+
-		+
-	}	+
-		+
-	~~.tabs li~~ {	+
-	~~background:white;~~	+
-	}	+
-		+
-	~~.tabs a,.tabs strong {~~	+
-	~~background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top;~~	+
-	~~color:white;~~	+
-	~~padding: 3px 10px 3px 4px;~~	+
-		+
-	}	+
-		+
-	~~.tabs strong{~~	+
-	~~color:#F20D0D;~~	+
-	~~background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");~~	+
-	}	+
-		+
-	~~.tabs a:hover{~~	+
-	~~color:red;~~	+
-	}	+
-		+
-		+
-	~~</style></html>~~	+
-		+
-	~~<div class="tabs-blue">~~	+
-	~~<ul>~~	+
-	~~<li>[[~~IGEM:IMPERIAL/2006/~~project/Bio elec interface\|~~Biosensor ~~Specifications]]</li>~~	+
-	~~<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Possible Implementation]]</li>~~	+
-	~~<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Correspondance with our contacts]]</li>~~	+
-	~~<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Our Sensor]]</li>~~	+
-	~~<li>[[IGEM:IMPERIAL/Protocols/Biosensortest\|Testing Protocol]]</li>~~	+
-	~~</ul>~~	+
-	~~</div>~~	+
-	~~<br style="clear:both">~~	+
-		+

	==Biological to Electrical Interface==		==Biological to Electrical Interface==

Cys at 11:05, 25 August 2006

Cys — Fri, 25 Aug 2006 11:05:16 GMT

←Older revision		Revision as of 11:05, 25 August 2006
Line 26:		Line 26:

	.tabs a:hover{		.tabs a:hover{
-	color:~~white~~;	+	color:red;
	}		}

Line 38:		Line 38:
	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Correspondance with our contacts]]</li>		<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Correspondance with our contacts]]</li>
	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Our Sensor]]</li>		<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Our Sensor]]</li>
		+	<li>[[IGEM:IMPERIAL/Protocols/Biosensortest\|Testing Protocol]]</li>
	</ul>		</ul>
	</div>		</div>

Cys at 23:25, 24 August 2006

Cys — Thu, 24 Aug 2006 23:25:52 GMT

←Older revision		Revision as of 23:25, 24 August 2006
Line 32:		Line 32:
	</style></html>		</style></html>

-	<div class="tabs">	+	<div class="tabs-blue">
	<ul>		<ul>
	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Biosensor Specifications]]</li>		<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Biosensor Specifications]]</li>

Cys at 23:21, 24 August 2006

Cys — Thu, 24 Aug 2006 23:21:02 GMT

←Older revision		Revision as of 23:21, 24 August 2006
Line 46:		Line 46:

	[[Image:Picture2.jpg\|thumb\|600px\|left\| System level diagram of biological to electrical interface]]		[[Image:Picture2.jpg\|thumb\|600px\|left\| System level diagram of biological to electrical interface]]
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+

	==Specifications==		==Specifications==

Cys: /* Biological to Electrical Interface */

Cys — Thu, 24 Aug 2006 23:18:08 GMT

Biological to Electrical Interface

←Older revision		Revision as of 23:18, 24 August 2006
Line 1:		Line 1:
-	=~~=Biological to Electrical Interface==~~	+	<html><style type='text/css'>
-		+	.tabs {
-	~~[[Image~~:~~Picture2.jpg\|thumb\|600px\|left\| System level diagram of biological to electrical interface]]~~	+	font-size:100%;
-		+	font-weight:none;
-		+	width: 100%;
-		+	color:white;
-		+	background:white;
-		+
-		+
-		+
-		+
-		+

		+	}

		+	.tabs li {
		+	background:white;
		+	}

		+	.tabs a,.tabs strong {
		+	background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top;
		+	color:white;
		+	padding: 3px 10px 3px 4px;

		+	}

		+	.tabs strong{
		+	color:#F20D0D;
		+	background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
		+	}

		+	.tabs a:hover{
		+	color:white;
		+	}


		+	</style></html>

		+	<div class="tabs">
		+	<ul>
		+	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Biosensor Specifications]]</li>
		+	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Possible Implementation]]</li>
		+	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Correspondance with our contacts]]</li>
		+	<li>[[IGEM:IMPERIAL/2006/project/Bio elec interface\|Our Sensor]]</li>
		+	</ul>
		+	</div>
		+	<br style="clear:both">


		+	==Biological to Electrical Interface==

		+	[[Image:Picture2.jpg\|thumb\|600px\|left\| System level diagram of biological to electrical interface]]

	==Specifications==		==Specifications==

Cys: /* Range of pH values we are expecting to see from different concentrations of AHL */

Cys — Thu, 24 Aug 2006 17:50:07 GMT

Range of pH values we are expecting to see from different concentrations of AHL

←Older revision		Revision as of 17:50, 24 August 2006
Line 270:		Line 270:
	*The acylase breaks down AHL into fatty acid (carboxylic acid) and HSL: AHL + H20 -> Fatty Acid + HSL		*The acylase breaks down AHL into fatty acid (carboxylic acid) and HSL: AHL + H20 -> Fatty Acid + HSL
	*Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.		*Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.
-	*pKa value found from [http://daecr1.harvard.edu/pdf/evans_pKa_table.pdf Harvard University: Evans Group pKa Table]: Carboxylic Acid with X=C6H5 -> ''' pKa=4.2'''	+	*pKa value found from [http://daecr1.harvard.edu/pdf/evans_pKa_table.pdf Harvard University: Evans Group pKa Table]: Carboxylic Acid with X=C6H5 -> ''' pKa = 4.2'''
	*Working out the Ka:		*Working out the Ka:
	**HA(aq) <-> H+ + A-		**HA(aq) <-> H+ + A-
	**Ka = [H+][A-]/[HA]		**Ka = [H+][A-]/[HA]
-	**pKa = -log(Ka) = 4.2 -> '''Ka=10E-4.2'''	+	**pKa = -log(Ka) = 4.2 -> '''Ka = 10E-4.2'''
		+	For [AHL] = 10E-9 M -> '''[H+] = 9.9910E-10 M'''
		+	*For [AHL] = 10E-3 M -> '''[H+] = 2.216 M'''

Cys: /* Range of pH values we are expecting to see from different concentrations of AHL */

Cys — Thu, 24 Aug 2006 17:47:46 GMT

Range of pH values we are expecting to see from different concentrations of AHL

←Older revision		Revision as of 17:47, 24 August 2006
Line 271:		Line 271:
	*Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.		*Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.
	*pKa value found from [http://daecr1.harvard.edu/pdf/evans_pKa_table.pdf Harvard University: Evans Group pKa Table]: Carboxylic Acid with X=C6H5 -> ''' pKa=4.2'''		*pKa value found from [http://daecr1.harvard.edu/pdf/evans_pKa_table.pdf Harvard University: Evans Group pKa Table]: Carboxylic Acid with X=C6H5 -> ''' pKa=4.2'''
		+	*Working out the Ka:
		+	**HA(aq) <-> H+ + A-
		+	**Ka = [H+][A-]/[HA]
		+	**pKa = -log(Ka) = 4.2 -> '''Ka=10E-4.2'''

Cys: /* Meeting with Dr. Danny O'Hare about pH sensor (18/8/2006) */

Cys — Thu, 24 Aug 2006 17:39:00 GMT

Meeting with Dr. Danny O'Hare about pH sensor (18/8/2006)

←Older revision		Revision as of 17:39, 24 August 2006
Line 262:		Line 262:

	See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Calendar/2006-8-18 Calendar 2006-8-18]		See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Calendar/2006-8-18 Calendar 2006-8-18]
		+
		+
		+	==Range of pH values we are expecting to see from different concentrations of AHL==
		+
		+	*Part C0061 produces AHL(N-acyl homoserine lactone), specifically it produces 3-oxohexanoyl-homoserine lactone (3OC6HSL)
		+	*We ordered the following enzyme, a generic acylase: [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A3010 Acylase I from porcine kidney]
		+	*The acylase breaks down AHL into fatty acid (carboxylic acid) and HSL: AHL + H20 -> Fatty Acid + HSL
		+	*Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.
		+	*pKa value found from [http://daecr1.harvard.edu/pdf/evans_pKa_table.pdf Harvard University: Evans Group pKa Table]: Carboxylic Acid with X=C6H5 -> ''' pKa=4.2'''