IGEM:IMPERIAL/2006/project/Bio elec interface: Difference between revisions
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{{IGEM:IMPERIAL/2006/Biosensor}} | |||
==Biological to Electrical Interface== | ==Biological to Electrical Interface== | ||
[[Image:Picture2.jpg|thumb|600px|left| System level diagram of biological to electrical interface]] | [[Image:Picture2.jpg|thumb|600px|left| System level diagram of biological to electrical interface]] | ||
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==Possible Implementation== | |||
#Signal transduction could involve (protein) redox-reactions | |||
#Antigen/Antibody sensors? | |||
#Protein sensors | |||
#Hormone/Neurotransmitter sensors (e.g. serotonin) | |||
:'''<u>Scenario 1:</u>''' | |||
*E.coli is in a liquid medium | |||
*Some bioprocess triggers production of a bio reporter (protein) in E.coli | |||
*The bio reporter is expelled from the cell into the medium | |||
*A sensor will measure the level of bio reporter (protein) in the medium | |||
:'''<u>Scenario 2:</u>''' | |||
*E.coli is in a liquid medium | |||
*Some bioprocess triggers productio of a bioreporter (protein) in E.coli | |||
*The bio reporter is expelled from the cell into the medium | |||
*There, the bio reporter (protein/enzyme) breaks down a certain chemical present in the medium | |||
*A commercially available sensor then measures the | |||
::a) increase in concentration of the breakdown products or, | |||
::b) the decrease in concentratio of the initial chemical | |||
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Latest revision as of 09:48, 30 October 2006
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Biological to Electrical Interface
Specifications
Bio Reporter | Signal Transducer |
---|---|
Expressed by E.coli | Detect Bio reporter |
Degradation in medium afterwards. Time needed for degradation should be equal to/less than that of GFP | Specific response to bio reporter only |
No other interaction with E.coli processes | Real-time readout |
Sufficient expression and secretion of bio reporter in medium for detection by signal transducer | Sufficient sensitivity to detect changes in bio reporter |
Commercially available |
Possible Implementation
- Signal transduction could involve (protein) redox-reactions
- Antigen/Antibody sensors?
- Protein sensors
- Hormone/Neurotransmitter sensors (e.g. serotonin)
- Scenario 1:
- E.coli is in a liquid medium
- Some bioprocess triggers production of a bio reporter (protein) in E.coli
- The bio reporter is expelled from the cell into the medium
- A sensor will measure the level of bio reporter (protein) in the medium
- Scenario 2:
- E.coli is in a liquid medium
- Some bioprocess triggers productio of a bioreporter (protein) in E.coli
- The bio reporter is expelled from the cell into the medium
- There, the bio reporter (protein/enzyme) breaks down a certain chemical present in the medium
- A commercially available sensor then measures the
- a) increase in concentration of the breakdown products or,
- b) the decrease in concentratio of the initial chemical
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