IGEM:IMPERIAL/2006/project/Bio elec interface: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
 
(35 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{IGEM:IMPERIAL/2006/Biosensor}}
==Biological to Electrical Interface==
==Biological to Electrical Interface==


[[Image:Picture2.jpg|thumb|600px|left| System level diagram of biological to electrical interface]]
[[Image:Picture2.jpg|thumb|600px|left| System level diagram of biological to electrical interface]]




Line 77: Line 73:




[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Calendar/2006-7-19 Summary of meeting with Professor Tony Cass]
<html>
 
<!-- Start of StatCounter Code -->
==Further Reading==
<script type="text/javascript" language="javascript">
var sc_project=1999441;
var sc_invisible=1;
var sc_partition=18;
var sc_security="18996820";
</script>


*[http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1985/57/i09/pdf/ac00286a027.pdf pH-based enzyme potentiometric sensors. Part 1. Theory]
<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img  src="http://c19.statcounter.com/counter.php?sc_project=1999441&amp;java=0&amp;security=18996820&amp;invisible=1" alt="website statistics" border="0"></a> </noscript>
*[http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1985/57/i09/pdf/ac00286a028.pdf pH-based enzyme potentiometric sensors. Part 2. Glucose-sensitive field effect transistor]
<!-- End of StatCounter Code -->
*[http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1985/57/i09/pdf/ac00286a029.pdf pH-based enzyme potentiometric sensors. Part 3. Penicillin-sensitive field effect transistor]
</html>

Latest revision as of 09:48, 30 October 2006

<html><style type='text/css'> .tabs { 

 font-size:100%;
 font-weight:none;
 width: 100%;
 color:white;
 background:white;

}

.tabs li { 

 background:white;

}

.tabs a,.tabs strong { 

 background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top;
 color:white;
 padding: 3px 10px 3px 4px;

}

.tabs strong{ 

 color:#F20D0D;
 background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");

}

.tabs a:hover{ 

 color:red;

}


</style></html>



Biological to Electrical Interface

System level diagram of biological to electrical interface









Specifications

Bio Reporter Signal Transducer
Expressed by E.coli Detect Bio reporter
Degradation in medium afterwards. Time needed for degradation should be equal to/less than that of GFP Specific response to bio reporter only
No other interaction with E.coli processes Real-time readout
Sufficient expression and secretion of bio reporter in medium for detection by signal transducer Sufficient sensitivity to detect changes in bio reporter
Commercially available



Possible Implementation

  1. Signal transduction could involve (protein) redox-reactions
  2. Antigen/Antibody sensors?
  3. Protein sensors
  4. Hormone/Neurotransmitter sensors (e.g. serotonin)


Scenario 1:
  • E.coli is in a liquid medium
  • Some bioprocess triggers production of a bio reporter (protein) in E.coli
  • The bio reporter is expelled from the cell into the medium
  • A sensor will measure the level of bio reporter (protein) in the medium


Scenario 2:
  • E.coli is in a liquid medium
  • Some bioprocess triggers productio of a bioreporter (protein) in E.coli
  • The bio reporter is expelled from the cell into the medium
  • There, the bio reporter (protein/enzyme) breaks down a certain chemical present in the medium
  • A commercially available sensor then measures the
a) increase in concentration of the breakdown products or,
b) the decrease in concentratio of the initial chemical


<html> <!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=1999441; var sc_invisible=1; var sc_partition=18; var sc_security="18996820"; </script>

<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&amp;java=0&amp;security=18996820&amp;invisible=1" alt="website statistics" border="0"></a> </noscript> <!-- End of StatCounter Code --> </html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:IMPERIAL/2006/project/Bio_elec_interface&oldid=83916"