IGEM:IMPERIAL/2006/Results
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Results
22/08/06
23/08/06
24/08/06
25/08/06
26/08/06
- The wrong AHL was used so we're discounting all data gathered on this day.
- All cultures for future testing are to be grown up from the freezer stock.
27/08/06
- The results of the T9002 assay are not useable...carry on testing.
- J37016 results look useable.
- J37020 testing done with wrong culture of J37020.
28/08/06
- The results of the T9002 assay are not useable...carry on testing.
- J37016 results look un-useable.
- J37020 testing done with wrong culture of J37020.
29/08/06
- No data collected today
30/08/06
- Data for T9002
- Data for J37016
- JS: Although not as promising as the 31/8 or 1/9 data, there is a definite trend that follows from other data collected on J37016. I would not venture to say that the data from this testing is conclusive, but when averaged with all the other data, I think we can manage to get a good transfer curve for our part.
- Data for J37020
31/08/06
- Data for T9002
- Data for J37016
- JS: I think we can use this set of data. It clearly shows a response between 1 to 10 nM and a peak fluorescence around 100 nM. Comparing this data to 30/8 data, there is definitely a more pronounced curve, however, as with all of the J37016 testing, there is a decrease in the fluorescence after 100 nM. This could be due to experimental error, as the error bars might suggest that the fluoescence stabilises around 2500 to 2600 units.
- Data for J37020
01/09/06
- Data for T9002
- Data for J37016
- JS: Having analysed this set of data, it looks like we can use it. As from the 31/8 data, we get a response about 1 to 10 nM and a peak around 100 nM. It is curious to note that there is a decrease in fluorescence as we further increase AHL concentration past the 100 nM mark, suggesting that there might be a possible problem with the AHL concentrations. Also, we are obtaining peak fluorescences of 2700 units which is consistent with 31/8 data.
- Data for J37020
02/09/06
- Data for T9002
- JS: These results look somewhat promising
- Data for J37016
- JS:
These results look somewhat promisingHaving looked at this set of data, there seems to be something wrong, as the OD of the cultures is only around 0.1. I don't think this is reasonable considering we left the plate in the shaker for at least 4 hours, and previous experimental data would suggest an OD of approximately 0.5 after 4 hours. I think we might have to discard this data. Any suggestions?
- JS:
- Data for J37020
- JS: I have to say...the results from this testing of J37020 isn't very promising
- JS: I will try to compile all of the J37016 data within the next few days. As I am busy with classes, I won't be able to guarantee anything, but I'll try my best. Also, MIT uses a linux system, which is great, but unfortunately, doesn't have any Microsoft software on it, and I am not too familiar with OpenOffice, so I have to bring in my laptop one day to download all of the data :(...However, I went to a talk by Drew Endy today and when he was showing a powerpoint slide of the iGEM wiki, I was very surprised to see Imperial College's picture on the front! At least I recognised all the people there. He did give a great talk which perhaps would have been better if we were all given it at the beginning of the project (since this talk was given mainly to 1st and 2nd year students who are interested in their Biological Engineering major here at MIT). None the less, it was great seeing you guys again, albeit in the most awkard place :)!
