IGEM:IMPERIAL/2006/LabCalendar/2006-8-26

• IGEM:IMPERIAL/2006/LabCalendar/2006-8-26 Events
• Day in the lab
• Project Progress

The Optical density mesuring device in the lab is broken !! Use Perkin Elmer Victor instead

Cryogenic Stocks

Grew up fresh day cultures for freezing at 9.30am

• T9002
• J37016
• J37015
• S01656
• J37015RS
• J37020

Using this protocol, we froze 6 different parts that we are testing

• Made up 10 Eppendorf tubes of S01656 with 15μL
• Made up 30 Eppendorf tubes of T9002 with 15μL
• Made up 25 Eppendorf tubes of J37015RS with 15μL
• Made up 20 Eppendorf tubes of J37015 with 15μL
• Made up 16 Eppendorf tubes of J37016 with 15μL
• Made up 20 Eppendorf tubes of J37020 with 15μL

• Transferred them to the -80[[:Category:{{{1}}}|{{{1}}}]] freezer (in Dr.Mann's section on 6th floor biochem.)

Testing

• Set up fresh cultures of T9002 and J37016, we didn't make a J37020 last night

JohnChattaway 05:15, 26 August 2006 (EDT)

Overnight culturing

• 2ml of T9002 for testing
• 2ml of J37016 for testing
• 2ml of J37020 for testing
• 2ml of J37024 (1) for miniprep
• 2ml of J37018 for miniprep
• 100ml of J37024 for maxiprep
• 100ml of J37018 for maxiprep
• 100ml of 4G for maxiprep