IGEM:IMPERIAL/2006/LabCalendar/2006-8-2

• IGEM:IMPERIAL/2006/LabCalendar/2006-8-2 Events
• Day in the lab
• Project Progress

M9 Broth has been made up and is sitting in the fridge in T/L

Electroporations

• We electroporated the following in the morning
  • I13521, 18I
  • 7A->3O DNA 2
• DNA and competent cells with DNA are in cold room
• Plates in 37C incubator in T/L

Maxiprep

• Maxi-preped the following parts
  • 1I
  • 5M
  • 12D->24A colony 1, 3, 4
  • 16P
  • 2M
  • 7A->3O colony 2
• Maxiprep DNA in fridge in T/L

Ligation

• Redo ligation 12D->2H
  • Cut 12D with P & X, yellow buffer
  • Cut 2H with S & P, orange buffer
• 30 uL digests
• extract DNA successful, however, there was a faint larger band in the 2H lane, but we took out the larger band which corresponded most closely to 3381 bp, the expected size
• Gene clean wash
• Set ligations at 14:30, to be ready at 16:30
• Gene clean ligation product
• Electroporation completed
• Incubated at 37C in Dr. Mann's Lab

Electrocompetent cells

• Cells have been made competent...see protocol