IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning by Standard Assembly /Entry Base: Difference between revisions
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[[Image:Red fluorescence image of pSB1C3-BBa_R0010-BBa_K516032 candidate colonies.jpg|500px|]]<br/> | [[Image:Red fluorescence image of pSB1C3-BBa_R0010-BBa_K516032 candidate colonies.jpg|500px|]]<br/> | ||
<b>Figure 2. Red fluorescence image of pSB1C3-BBa_R0010-BBa_K516032 candidate colonies.</b> Transformants of ligation product of pSB1C3-BBa_R0010-BBa_K516032 were observed under fluorescence macroscope for signs of mRFP. Transformants were first incubated at 37°C for 24 hours, followed by storage at 4°C for 20 hours before observation. Bright field (BF) images were taken with 50 ms exposure and gain 1 acquisition while 500 ms exposure and gain 2 acquisition were used for red fluorescence (RFP) images. Scale bar = 5 mm. | <b>Figure 2. Red fluorescence image of pSB1C3-BBa_R0010-BBa_K516032 candidate colonies.</b> Transformants of ligation product of pSB1C3-BBa_R0010-BBa_K516032 were observed under fluorescence macroscope for signs of mRFP. Transformants were first incubated at 37°C for 24 hours, followed by storage at 4°C for 20 hours before observation. Bright field (BF) images were taken with 50 ms exposure and gain 1 acquisition while 500 ms exposure and gain 2 acquisition were used for red fluorescence (RFP) images. D, E, J and N are replicates prepared by Daniel, Edward, Jenny and Natalie repectively. Scale bar = 5 mm. | ||
Revision as of 10:07, 1 February 2016