IGEM:Hong Kong HKUST/Investigations: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→2016) |
(→2016) |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 33: | Line 33: | ||
==2016== | ==2016== | ||
*[[IGEM:Hong Kong HKUST/Investigations/ Effect of dephosphorylation in reducing the background ligation | Effect of dephosphorylation in reducing the background ligation]] | |||
*[[IGEM:Hong Kong HKUST/Investigations/ Effects of vector to insert DNA ratio on ligation efficiency | Effects of vector to insert DNA ratio on ligation efficiency]] | |||
*[[IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning by Standard Assembly /Entry Base | Plasmid Cloning by Standard Assembly]] | *[[IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning by Standard Assembly /Entry Base | Plasmid Cloning by Standard Assembly]] | ||
*[[IGEM:Hong Kong HKUST/Investigations/ Restriction Enzyme Activities At 4°C,37°C and 65°C | Restriction Enzyme Activities At 4°C,37°C and 65°C]] | *[[IGEM:Hong Kong HKUST/Investigations/ Restriction Enzyme Activities At 4°C,37°C and 65°C | Restriction Enzyme Activities At 4°C,37°C and 65°C]] | ||
*[[IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning of BFP Generator Using Standard Assembly | Plasmid Cloning of BFP Generator Using Standard Assembly]] | |||
*[[IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning of GFP Generator Using Standard Assembly | Plasmid Cloning of GFP Generator Using Standard Assembly]] | |||
*[[IGEM:Hong Kong HKUST/Investigations/ Identifying Candidate Colonies by Colony PCR | Identifying Candidate Colonies by Colony PCR]] | |||
Revision as of 09:44, 29 May 2016
List of Investigations:
(Sorted by alphabetic order)
2014
- Comparison Between WN Buffer & 70% Ethanol in Viogene Miniprep Kit
- Comparison of Different Growth Media for Plasmid Production
- Effect of Agarose Concentrations on Separation of Linear DNA in Gel Electrophoresis
- Effect of ATP Concentrations on Ligation Efficiency
- Performance of NotI-HF in 5, 15 and 30 minutes
- Recovery Time and Transformation Efficiency
- Restriction Enzyme Activities at 37°C vs. 20°C
- Robustness of Colony PCR Compared to Regular PCR
2015
- Investigation on the Functionality of RNA Thermometers
- Investigation on the Light-Sensitive amilCP Generator
- Effect of overloading cells on growth rate
- Effect of Dephosphorylation on Reduction of Background Ligation
- Identifying candidate colonies in molecular level
- Effects of ATP concentration to the Ligation Efficiency
- Plasmid Cloning by Restriction Enzyme Digestion
- The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation
2016
- Effect of dephosphorylation in reducing the background ligation
- Effects of vector to insert DNA ratio on ligation efficiency
- Plasmid Cloning by Standard Assembly
- Restriction Enzyme Activities At 4°C,37°C and 65°C
- Plasmid Cloning of BFP Generator Using Standard Assembly
- Plasmid Cloning of GFP Generator Using Standard Assembly
- Identifying Candidate Colonies by Colony PCR
