IGEM:Hong Kong HKUST/Investigations

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List of Investigations:
(Sorted by alphabetic order)

2014

• Comparison Between WN Buffer & 70% Ethanol in Viogene Miniprep Kit
• Comparison of Different Growth Media for Plasmid Production
• Effect of Agarose Concentrations on Separation of Linear DNA in Gel Electrophoresis
• Effect of ATP Concentrations on Ligation Efficiency
• Performance of NotI-HF in 5, 15 and 30 minutes
• Recovery Time and Transformation Efficiency
• Restriction Enzyme Activities at 37°C vs. 20°C
• Robustness of Colony PCR Compared to Regular PCR

2015

• Investigation on the Functionality of RNA Thermometers
• Investigation on the Light-Sensitive amilCP Generator
• Effect of overloading cells on growth rate
• Effect of Dephosphorylation on Reduction of Background Ligation
• Identifying candidate colonies in molecular level
• Effects of ATP concentration to the Ligation Efficiency
• Plasmid Cloning by Restriction Enzyme Digestion
• The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation

2016

• Effect of dephosphorylation in reducing the background ligation
• Effects of vector to insert DNA ratio on ligation efficiency
• Plasmid Cloning by Standard Assembly
• Restriction Enzyme Activities At 4°C,37°C and 65°C
• Plasmid Cloning of BFP Generator Using Standard Assembly
• Plasmid Cloning of GFP Generator Using Standard Assembly
• Identifying Candidate Colonies by Colony PCR