IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions

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==Results and Interpretations==
==Results and Interpretations==
[[Image:OD 600 project.jpeg|1080px|center]]<br><br>
[[Image:OD 600 project.jpeg|900px|center]]<br><br>


==Discussion==
==Discussion==

Revision as of 00:08, 28 January 2015

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Authors

  • TANG, Chloe T.C.
  • Tam, L.F.
  • Tam, K.M.
  • Tang, Mandy L.Y.
  • Siu, Mona M.Y.
  • Yiu, Stephanie P.T.

Abstract

It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome binding site (BBa_I13003: Registry of Standard Biological Parts) in Escherichia .coli to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the effect of overloading cells on growth rate was insignificant.

Introduction

Methods and Materials

Results and Interpretations



Discussion

In our hypothesis, it is expected that strong gene expression will affect the growth and viability of cells. However, our results shows no differences between the experimental setup with strong gene expression and the control in terms of its growth rate. One possible explanation is that the promoter (BBa_J23101) and Ribosome Binding Site (BBa_B0032) that we have chosen to use in our experimental might not strong enough. Overloading of cells can not be generated. Therefore, their results shows similar pattern.

Conclusion

Both the experimental cells and the control show a similar growth pattern and growth rate. It is concluded that either the experimental setup does not contain a strong enough promoter and ribosome binding site to create an environment of overloading cells; or overloading of cell have insignificant effect on its growth rate.



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