IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions

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* Phoebe Tam
* Phoebe Tam
* Tam, K.M.
* Tam, K.M.
* Mandy Tang
* Tang, Mandy L.Y.
* Mona Siu
* Mona Siu
* Yiu, Stephanie P.T.
* Yiu, Stephanie P.T.

Revision as of 22:53, 27 January 2015

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Authors

Abstract

It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome binding site (BBa_I13003: Registry of Standard Biological Parts) in Escherichia .coli to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the effect of overloading cells on growth rate was insignificant.

Introduction

Methods and Materials

Results and Interpretations

Discussion

Conclusion
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