IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions

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==Abstract==
==Abstract==
It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) and a meidum ribosome bonding site (BBa_I13003: Registry of Standard Biological Parts) in <em>Escherichia .coli</em> to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the growth and viability of cells were not affected by strong gene expression.
It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome bonding site (BBa_I13003: Registry of Standard Biological Parts) in <em>Escherichia .coli</em> to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the growth and viability of cells were not affected by strong gene expression.


==Introduction==
==Introduction==

Revision as of 22:49, 27 January 2015

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Authors

Abstract

It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome bonding site (BBa_I13003: Registry of Standard Biological Parts) in Escherichia .coli to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the growth and viability of cells were not affected by strong gene expression.

Introduction

Methods and Materials

Results and Interpretations

Discussion

Conclusion