IGEM:Harvard/2010/Plasmids: Difference between revisions
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these plasmids currently contain multiple cloning sites (MCSs) with many, many restriction enzyme cut sites. we will need to change the MCSs such that they are biobrick compatible. <br/> | these plasmids currently contain multiple cloning sites (MCSs) with many, many restriction enzyme cut sites. we will need to change the MCSs such that they are biobrick compatible. <br/> | ||
[[ | [[Media:pORE_O1.gb|pORE_O1]]<br/> | ||
[[ | [[Media:pORE_O2.gb|pORE_O2]]<br/> | ||
[[ | [[Media:PORE_E3.gb|pORE_E3]]<br/> | ||
[[ | [[Media:PORE_E4.gb|pORE_E4]]<br/> | ||
[[ | [[Media:PORE_R1.gb|pORE_R1]]<br/> | ||
[[Media:PORE_R3.gb|pORE_R3]]<br/> | |||
[[Media:V7.gb|V7: pORE_O1 biobricked]]<br/> | |||
[[Media:V8.gb|V8: pORE_O2 biobricked]]<br/> | |||
[[Media:V9.gb|V9: pORE_E3 biobricked]]<br/> | |||
[[Media:V10.gb|V10: pORE_E4 biobricked]]<br/> | |||
[[Media:V11.gb|V11: pORE_R1 biobricked]]<br/> | |||
[[Media:V12.gb|V12: pORE_R3 biobricked]]<br/> | |||
Latest revision as of 20:58, 21 June 2010
the plant vectors we will be biobrick-ing and using to transform into arabidopsis come from this paper:
Coutu, et al. "pORE: a modular binary vector series suited for both monocot and dicot plant transformation" Transgenic Res (2007) 16: 771-781.
this paper established a whole slew of vectors with different plant promoters and antibiotic resistance markers.
we have ordered the following vectors: O1, O2, E3, E4, R1, and R3.
these plasmids currently contain multiple cloning sites (MCSs) with many, many restriction enzyme cut sites. we will need to change the MCSs such that they are biobrick compatible.
pORE_O1
pORE_O2
pORE_E3
pORE_E4
pORE_R1
pORE_R3
V7: pORE_O1 biobricked
V8: pORE_O2 biobricked
V9: pORE_E3 biobricked
V10: pORE_E4 biobricked
V11: pORE_R1 biobricked
V12: pORE_R3 biobricked
