IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/06: Difference between revisions

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==Team Allergy==
==Team Allergy==
'''amiRNA'''
* amiRNA-transformed turbo cells didn't grow in YEB medium. There should be more LB+Amp available by the end of the day. In the meantime, we're digesting some more backbone (8x20ul X+P+B21)
* amiRNA-transformed turbo cells didn't grow in YEB medium. There should be more LB+Amp available by the end of the day. In the meantime, we're digesting some more backbone (8x20ul X+P+B21)
'''ihpRNA'''
*no colonies on plates from transformations done yesterday after 16 hours (we let these plates grow for longer)
*redoing ligations of ihpRNA inserts in V9/V10
**Redigested and gel purified some backbone




Revision as of 08:22, 6 August 2010

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Team Allergy

amiRNA

  • amiRNA-transformed turbo cells didn't grow in YEB medium. There should be more LB+Amp available by the end of the day. In the meantime, we're digesting some more backbone (8x20ul X+P+B21)

ihpRNA

  • no colonies on plates from transformations done yesterday after 16 hours (we let these plates grow for longer)
  • redoing ligations of ihpRNA inserts in V9/V10
    • Redigested and gel purified some backbone



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