IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/02: Difference between revisions
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** Digested J45004, J45017, and B21 BB with XbaI/PstI | ** Digested J45004, J45017, and B21 BB with XbaI/PstI | ||
* Gel extracted and purified bands indicated in image below | * Gel extracted and purified bands indicated in image below | ||
* Ligated inserts into 50ng of B21 v0120 backbone with a 3:1 insert to backbone ratio | * Ligated inserts into 50ng of B21 v0120 backbone with a 3:1 insert to backbone ratio |
Revision as of 06:45, 3 August 2010
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Team FenceGel extractionGal4 PCR product and Ec digest gel extract and purified. Gal on the left, EcR on the right VP16 PCR 2No PCR product is visible
Team FlavorDTL of Mira/Brazz+StrepII+Stop into V24
DTL of Wintergreen pathway
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