IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/26: Difference between revisions
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*Ran PCR product of Valencia Orange genome to confirm: <font color="red">failed</font> | *Ran PCR product of Valencia Orange genome to confirm: <font color="red">failed</font> | ||
[[Image:StrepStopNotSpeValPCR.jpg|240px]] [[Image:StrepStopNotSpeValPCR2.jpg|240px]] | [[Image:StrepStopNotSpeValPCR.jpg|240px]] [[Image:StrepStopNotSpeValPCR2.jpg|240px]] | ||
*Digested Mira/Brazz+StrepII constructs EcoRI/SpeI, STOP+V0120 EcoRI/XbaI | *Digested Mira/Brazz+StrepII constructs EcoRI/SpeI, STOP+V0120 EcoRI/XbaI |
Revision as of 13:01, 26 July 2010
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Team FencePFX polymerase PCR
9.5μL total DNA: 21ng/μL
9.5μL + 4μL = 13.5μL therefore 6.5μL H20 per reaction MM
total volume:100μL Team Allergy
Team Flavor
Digestion Reactions Mira N: 888ng Mira C: 888ng Brazz N: 993ng Brazz C: 1032ng STOP: 781ng x 2 (max DNA available)
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