IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
==Team Flavor==
==Team Flavor==
* DTL of Mira/Brazz+StrepII into STOP+V0120
===DTL of Mira/Brazz+StrepII into STOP+V0120===
* Confirm of valencene PCR (failed)
 
''Digestion''
 
{| border="1"
|+ ''Digestion Reactions''
!  !! Stop v0120 1!! Stop v0120 2
|-
! DNA
| align="center" |10|| align="center" |  8
|-
! FD Buffer (10x)
| align="center"  | 2 || align="center" |  2
|-
! diH<sub>2</sub>O
| align="center"  | 6 || align="center" |  8
|-
! Xba1
| align="center"  | 1 || align="center" |  1
|-
! EcoRI
| align="center" | 1 || align="center" |  1
|-
|}
 
==Confirm of valencene PCR (failed)==
 
[[Image:BackbonePCRconfirm.jpg|240px]]
[[Image:BackbonePCRconfirm.jpg|240px]]


Revision as of 07:22, 20 July 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Team Flavor

DTL of Mira/Brazz+StrepII into STOP+V0120

Digestion

Digestion Reactions
Stop v0120 1 Stop v0120 2
DNA 10 8
FD Buffer (10x) 2 2
diH2O 6 8
Xba1 1 1
EcoRI 1 1

Confirm of valencene PCR (failed)

  • Re-do of Valencene PCR (failed)

Team Allergy

  • Got back sequencing results of GerS, BetS, PDK
    • All three samples of GerS sent in worked and PDK worked
  • Ran PCR of Friday's digested B21. The gel suggested a partial digest: we saw two bands of backbone where we should have seen only one:
  • Sending 6 samples of amiRNA (LTP.5), 5 samples of amiRNA (LTP), 6 samples of amiRNA (Bet)
  • Second digest of B21 appears to have been more successful:
  • Ligating and transforming backbone + allergen parts
  • Insert your content here.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/07/19&oldid=434276"