IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/14: Difference between revisions
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# 1kb Plus Ladder | # 1kb Plus Ladder | ||
''Ligation of Mira/Brazz+Strep and STOP codon BioBrick'' <br/> | |||
{| border="1" | |||
|+ ''Ligation Reactions'' | |||
! !! Mira StrepII N + STOP w/ v0120 BB !! Mira StrepII C + STOP w/ v0120 BB !! Brazz StrepII N + STOP w/ v0120 BB !! Brazz StrepII C + STOP w/ v0120 BB !! Control STOP w/ v0120 BB | |||
|- | |||
! diH<sub>2</sub>O | |||
| align="center" | 4.5 || align="center" | 4.5 || align="center" | 9 || align="center" | 5 || align="center" | 12 | |||
|- | |||
! T4 DNA ligase buffer (10x) | |||
| align="center" | 2 || align="center" | 3 || align="center" | 2 || align="center" | 2 || align="center" | 2 | |||
|- | |||
! DNA Insert | |||
| align="center" | 7.5 || align="center" | 7.5 || align="center" | 2 || align="center" | 6 || align="center" | 0 | |||
|- | |||
! DNA Backbone | |||
| align="center" | 5 || align="center" | 5 || align="center" | 6 || align="center" | 6 || align="center" | 5 | |||
|- | |||
! T4 DNA ligase | |||
| align="center" | 1 || align="center" | 1|| align="center" | 1|| align="center" | 1|| align="center" | 1 | |||
|- | |||
|} | |||
''Transformation'' | |||
* Transformed 5 μL ligation mix with 15 μL TURBO e. coli cells | |||
* Plated on LB + Amp plates and left in 37°C incubator overnight | |||
==Team Fence== | ==Team Fence== | ||
Revision as of 12:55, 14 July 2010
iGEM iGarden | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Team FlavorMiniprep of STOP + V0120 partNanodrop O/D's: STOP V0120 1-1: 134.4 STOP V0120 1-2: 98.7 STOP V0120 2-1: 55.2 STOP V0120 2-2: 121.8 DTL of StrepII+Mira/Brazz with STOP codon
Digestion of Mira/Brazz+Strep and STOP codon BioBrick
Team FencePCR of the arabidopsis promoters with the newly extracted DNA and the older DNA. FAIL Arp2 promoter PCR failed. Exp2 promoter PCR failed. Phenol Chloroform DNA Extraction
THE END |