IGEM:Harvard/2008/Strains

Yeah, this will need some reorganization...

Strains

• S1: Shewanella oneidensis MR-1
  • Codon usage
  • Shewanella oneidensis 16s rRNA
  • Colonies go slightly pink as they reach maturity. Pellets of overnights are pink. Is prob. high heme content in anaerobic conditions.
• S2: Shewanella putrefaciens CN-32
  • Codon usage
  • ATCC page - growth conditions
• S3: Shewanella oneidensis MR-1 ΔmtrA
• S4: Shewanella oneidensis MR-1 ΔmtrB
• S5: Snewanella oneidensis MR-1 ΔmtrC/omcA
• E1: Escherichia coli DH5α
  • 16s rRNA (for DH10B)
• E2: Escherichia coli TOP10
• E3: Escherichia coli DB31 (ccDB res) (death-resistant strain)
• E4: RU1012 [MC4100 ara+ Φ(OmpC-lacZ) 10-25 ΔenvZ::KanR]
• E5: XL1-Blue (from QuikChange kit)

Growth conditions

• /E coli/
• /Shewie/