IGEM:Harvard/2008/Strains: Difference between revisions
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** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=211586 Codon usage] | ** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=211586 Codon usage] | ||
**[[Shewanella oneidensis 16s rRNA]] | **[[Shewanella oneidensis 16s rRNA]] | ||
**Colonies go slightly pink as they reach maturity. Pellets of overnights are pink. Is prob. high heme content in anaerobic conditions. | |||
* S2: ''Shewanella putrefaciens'' CN-32 | * S2: ''Shewanella putrefaciens'' CN-32 | ||
** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=319224 Codon usage] | ** [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=319224 Codon usage] | ||
** [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=700550&Template=bacteria ATCC page - growth conditions] | ** [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=700550&Template=bacteria ATCC page - growth conditions] | ||
* S3: ''Shewanella oneidensis MR-1'' ΔmtrA | |||
* S4: ''Shewanella oneidensis MR-1'' ΔmtrB | |||
* S5: ''Snewanella oneidensis MR-1'' ΔmtrC/omcA | |||
* E1: Escherichia coli DH5α | * E1: Escherichia coli DH5α | ||
**[[16s rRNA (for DH10B)]] | **[[16s rRNA (for DH10B)]] | ||
* E2: Escherichia coli TOP10 | * E2: Escherichia coli TOP10 | ||
* E3: Escherichia coli DB31 (ccDB res) (death-resistant strain) | |||
* E4: RU1012 [MC4100 ara+ Φ(OmpC-lacZ) 10-25 ΔenvZ::KanR] | |||
* E5: XL1-Blue (from QuikChange kit) | |||
== '''Growth conditions''' == | == '''Growth conditions''' == | ||
* [[/E coli/]] | * [[/E coli/]] | ||
* [[/Shewie/]] | * [[/Shewie/]] |
Latest revision as of 17:29, 13 August 2008
Yeah, this will need some reorganization...
Strains
- S1: Shewanella oneidensis MR-1
- Codon usage
- Shewanella oneidensis 16s rRNA
- Colonies go slightly pink as they reach maturity. Pellets of overnights are pink. Is prob. high heme content in anaerobic conditions.
- S2: Shewanella putrefaciens CN-32
- S3: Shewanella oneidensis MR-1 ΔmtrA
- S4: Shewanella oneidensis MR-1 ΔmtrB
- S5: Snewanella oneidensis MR-1 ΔmtrC/omcA
- E1: Escherichia coli DH5α
- E2: Escherichia coli TOP10
- E3: Escherichia coli DB31 (ccDB res) (death-resistant strain)
- E4: RU1012 [MC4100 ara+ Φ(OmpC-lacZ) 10-25 ΔenvZ::KanR]
- E5: XL1-Blue (from QuikChange kit)