IGEM:Harvard/2008/Plasmids
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Vectors
Parts
| Name | Registry Name | Description | Origin | Size | Marker | Transformed? | Miniprepped? | Culture in Glycerol Stock? |
| P1 | J23113/I20259 | Low promoter GFP tester | p15A pMR101-derived | 919 | Kan | in S1 and TOP10 | Yes for both strains | Yes for both strains. |
| P2 | J23150/I20269 | Medium promoter GFP tester | p15A pMR101-derived | 919 | Kan | in TOP10, not S1 | ||
| P3 | J23151/I20270 | High promoter GFP tester | p15A pMR101-derived | 919 | Kan | in TOP10 and S1 | Yes for both strains. | Yes for both strains. |
| P4 | pACYC duet | Low-Medium copy vector | p15a | 4008 | Cm | Yes | in S1 and TOP10 | |
| P5 | pSB3K3 | Low-Medium copy vector (w/ death gene) | p15a | 2750 | Kan | |||
| P6 | BBa-E1010 | RFP only (w/ death gene) | pMB1 | 681 | Kan | |||
| P7 | pSB1A2 | GFP only | pMB1 | 2079 | Amp | |||
| P8 | BBa_J04450 | RFP with LacI promoter | pMB1 | 1069 | Amp | |||
| P9 | BBa_J04430 | GFP with LacI promoter | pMB1 | 1083 | Amp | |||
| P10 | BBa_I715038 | T7 Polymerase with LacI promoter | pMB1 | 2878 | Amp | |||
| P11 | BBa_M30109 | Light responsive system, dual regulation | pUC19-derived pMB1 | 4333 | Amp/Cm | |||
| P12 | pETDuet-1 | Low copy plasmid | pBR322-derived ColE1 | 5420 | Amp | |||
| P13 | pCDFDuet-1 | Low copy plasmid | CloDF13 | 3781 | Sm | Yes | in S1 | |
| P14 | pCOLADuet-1 | Low copy plasmid | ColA | 3719 | Kan | |||
| P15 | BBa_I13522 | GFP with Tet promoter | ColE1 | 937 | Amp | |||
| P16 | BBa_R0040 | Tet repressible promoter | pMB1 | 54 | Amp | |||
| P17 | BBa_Q04400 | Inverter (TetR w/o promoter and Tet promoter) | OriS, P1 lytic, F1 | 902 | Kan | |||
| P18 | BBa_R0051 | lambda promoter (cI regulated) | pMB1 | 49 | Amp | |||
| P19 | BBa_I763007 | RFP with lambda promoter | pMB1 | 918 | Amp | |||
| P20 | BBa_J04431 | GFP (LVA tagged) with Lac promoter | pMB1 | 1122 | Amp, Kan | |||
| P21 | A1 (pACYC) | pACYC (P4) w/ biobricks | ??? | ??? | ??? | Yes | in S1 and TOP10 | |
| P22 | BBa_R0082 | ompR controlled promoter for ompC (long) | pMB1 | 108 | Amp | |||
| P23 | BBa_R0083 | ompR controlled promoter for ompC (short) | pMB1 | 78 | Amp | |||
| P24 | BBa_R0084 | ompR controlled promoter for ompF | pMB1 | 108 | Amp | |||
| P25 | BBa_J58102 | Promoter activated by OmpR-P with the reporter GFP | pUC19-derived pMB1 | 991 | Amp | |||
| P26 | BBa_J58103 | Mutated promoter activated by OmpR-P with the reporter GFP | pUC19-derived pMB1 | 961 | Amp | |||
| P27 | pZS4int2 | repressor plasmid | pSC101 | Sm | Yes | |||
| P28 | pZE21 | GFP | ColE1 | Kan | Yes | |||
| P29 | pZS*2R | Venus (mutant YFP) | pSC101* | Kan | Yes | |||
| P30 | pZA32 | YFP | p15A | Cm | Yes | |||
| P31 | pZS*1R | Venus | pSC101* | Amp | Yes | |||
| P32 | pZA32 | Venus | p15A | Cm | Yes | |||
| P33 | pZA31 | Venus | p15A | Cm | Yes | |||
| P34 | pZE12 | GFP | ColE1 | Amp | Yes | |||
| P35 | pLDR8 | Yes | ||||||
| P36 | BBa_I763004 | GFP + IPTG + LVA | Kan/Amp | |||||
| P37 | BBa_I722007 | Constructive expression LacI w/ pTetR promoter | Amp | |||||
| P38 | BBa_J23114 | High constitutive promoter | Amp | |||||
| P39 | BBa_J23113 | Low constitutive promoter | Amp | |||||
| P40 | BBa_B0032 | Ribosome Binding Site | Amp | |||||
| P41 | BBa_B0010 | Transcriptional Terminator | Amp | |||||
| P42 | BBa_C0012 | LacI coding region | Amp | |||||
| P43 | [1] | TetR coding region | Amp | |||||
| P44 | BBa_C0051 | cI lambda | Amp | |||||
| P45 | BBa_E0240 | GFP only w/ RBS & terminator | Amp | |||||
| P46 | BBa_I51020 | Base Vector | Amp | |||||
| P47 | BBa_P1003 | Kan Resistance Cassette | Kan | |||||
| P48 | BBa_P1004 | Cm Resistance Cassette | Cm | |||||
| P49 | BBa_P1002 | Amp Resistance Cassette | Amp | |||||
| P50 | BBa_P1005 | Tet Resistance Cassette | Tet |
