IGEM:Harvard/2008/Plasmids

Vectors

• pACYC-Duet vector map
• pSB3K3

Parts

• Google Spreadsheet

Name	Registry Name	Description	Origin	Size	Marker	Transformed?	Miniprepped?	Culture in Glycerol Stock?	Sequenced?
P1	J23113/I20259	Low promoter GFP tester	p15A pMR101-derived	919	Kan	in S1 and TOP10	Yes for both strains	Yes for both strains.
P2	J23150/I20269	Medium promoter GFP tester	p15A pMR101-derived	919	Kan	in TOP10, not S1
P3	J23151/I20270	High promoter GFP tester	p15A pMR101-derived	919	Kan	in TOP10 and S1	Yes for both strains.	Yes for both strains.
P4	pACYC duet	Low-Medium copy vector	p15a	4008	Cm	Yes	in S1 and TOP10
P5	pSB3K3	Low-Medium copy vector (w/ death gene)	p15a	2750	Kan
P6	BBa-E1010	RFP only (w/ death gene)	pMB1	681	Kan
P7	pSB1A2	GFP only	pMB1	2079	Amp
P8	BBa_J04450	RFP with LacI promoter	pMB1	1069	Amp
P9	BBa_J04430	GFP with LacI promoter	pMB1	1083	Amp
P10	BBa_I715038	T7 Polymerase with LacI promoter	pMB1	2878	Amp
P11	BBa_M30109	Light responsive system, dual regulation	pUC19-derived pMB1	4333	Amp/Cm
P12	pETDuet-1	Low copy plasmid	pBR322-derived ColE1	5420	Amp
P13	pCDFDuet-1	Low copy plasmid	CloDF13	3781	Sm	Yes	in S1
P14	pCOLADuet-1	Low copy plasmid	ColA	3719	Kan
P15	BBa_I13522	GFP with Tet promoter	ColE1	937	Amp
P16	BBa_R0040	Tet repressible promoter	pMB1	54	Amp
P17	BBa_Q04400	Inverter (TetR w/o promoter and Tet promoter)	OriS, P1 lytic, F1	902	Kan
P18	BBa_R0051	lambda promoter (cI regulated)	pMB1	49	Amp
P19	BBa_I763007	RFP with lambda promoter	pMB1	918	Amp
P20	BBa_J04431	GFP (LVA tagged) with Lac promoter	pMB1	1122	Amp, Kan
P21	A1 (pACYC)	pACYC (P4) w/ biobricks	???	???	???	Yes	in S1 and TOP10
P22	BBa_R0082	ompR controlled promoter for ompC (long)	pMB1	108	Amp
P23	BBa_R0083	ompR controlled promoter for ompC (short)	pMB1	78	Amp
P24	BBa_R0084	ompR controlled promoter for ompF	pMB1	108	Amp
P25	BBa_J58102	Promoter activated by OmpR-P with the reporter GFP	pUC19-derived pMB1	991	Amp
P26	BBa_J58103	Mutated promoter activated by OmpR-P with the reporter GFP	pUC19-derived pMB1	961	Amp
P27	pZS4int2	repressor plasmid	pSC101		Sm	Yes
P28	pZE21	GFP	ColE1		Kan	Yes
P29	pZS*2R	Venus (mutant YFP)	pSC101*		Kan	Yes
P30	pZA32	YFP	p15A		Cm	Yes
P31	pZS*1R	Venus	pSC101*		Amp	Yes
P32	pZA32	Venus	p15A		Cm	Yes
P33	pZA31	Venus	p15A		Cm	Yes
P34	pZE12	GFP	ColE1		Amp	Yes
P35	pLDR8					Yes
P36	BBa_I763004	GFP + IPTG + LVA			Kan/Amp
P37	BBa_I722007	Constructive expression LacI w/ pTetR promoter			Amp
P38	BBa_J23114	High constitutive promoter			Amp
P39	BBa_J23113	Low constitutive promoter			Amp
P40	BBa_B0032	Ribosome Binding Site			Amp
P41	BBa_B0010	Transcriptional Terminator			Amp
P42	BBa_C0012	LacI coding region			Amp
P43	[1]	TetR coding region			Amp
P44	BBa_C0051	cI lambda			Amp
P45	BBa_E0240	GFP only w/ RBS & terminator			Amp
P46	BBa_I51020	Base Vector			Amp
P47	BBa_P1003	Kan Resistance Cassette			Kan
P48	BBa_P1004	Cm Resistance Cassette			Cm
P49	BBa_P1002	Amp Resistance Cassette			Amp
P50	BBa_P1005	Tet Resistance Cassette			Tet
P51	BBa_Q04121	LacI QPI w/ RBS & promoter			Kan
P52	BBa_Q04510	cI Lambda QPI			Kan
P53	BBa_P0440	PoPS -> TetR			Amp
P54	BBa_P0412	PoPS -> LacI			Amp
P55	BBa_P0455	RBS.cI.LVA.Term			Amp/Cm