IGEM:Harvard/2008/Lab Notebooks/Recipes: Difference between revisions
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==Orianna's growth media== | ==Orianna's growth media== | ||
* 50 mM K-PIPES 18.925 g/L | * 50 mM K-PIPES '''18.925 g/L''' | ||
* 7.5 mM NaOH 7.5 mL/L | * 7.5 mM NaOH '''7.5 mL/L''' | ||
* 28 mM NH<sub>4</sub>Cl 1.5 g/L | * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' | ||
* 1.3 mM KCl 433 uL/L | * 1.3 mM KCl '''433 uL/L''' | ||
* 4.3 mM NaH<sub>2</sub>PO<sub>4</sub> .59 g/L | * 4.3 mM NaH<sub>2</sub>PO<sub>4</sub> '''.59 g/L''' | ||
* 100 mM NaCl 5.84 g/L | * 100 mM NaCl '''5.84 g/L''' | ||
* 18 mM Lactate 1.62 g/L | * 18 mM Lactate '''1.62 g/L''' | ||
* 10 mL Vitamin solution | * 10 mL Vitamin solution | ||
* 10 mL Amino acid solution | * 10 mL Amino acid solution |
Revision as of 08:33, 15 July 2008
Recipes
Orianna's growth media
- 50 mM K-PIPES 18.925 g/L
- 7.5 mM NaOH 7.5 mL/L
- 28 mM NH4Cl 1.5 g/L
- 1.3 mM KCl 433 uL/L
- 4.3 mM NaH2PO4 .59 g/L
- 100 mM NaCl 5.84 g/L
- 18 mM Lactate 1.62 g/L
- 10 mL Vitamin solution
- 10 mL Amino acid solution
- 10 mL Trace mineral solution
LML medium (for anaerobic Shewanella growth)
- 0.02% yeast extract
- 0.01% peptone
- 20 mM lactate
- 10 mM HEPES [pH 7.4]
LML agar (for Shewanella reduction test)
- LML medium
- 0.8% agar
- 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))
(LML recipes from Beliaev and Saffarini 1998)
Chamber media
- 5.844 g/L 100mM NaCl
- 15.1185 g/L 50mM PIPES (hydrogen)
7.0 pH
Phosphate buffer
- 2.918 g/L Monosodium phosphate, monohydrate
- 4.095 g/L Disodium phosphate, anhydrous
- 5.844 g/L 100mM NaCl
7.0 pH