IGEM:Harvard/2008/Lab Notebooks/Recipes: Difference between revisions
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=Recipes= | =Recipes= | ||
==LML medium (for anaerobic Shewanella growth)== | ==LML medium (for anaerobic Shewanella growth)== | ||
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* 20 mM lactate | * 20 mM lactate | ||
* 10 mM HEPES [pH 7.4] | * 10 mM HEPES [pH 7.4] | ||
==LML agar (for Shewanella reduction test)== | ==LML agar (for Shewanella reduction test)== | ||
* LML medium | * LML medium | ||
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(LML recipes from [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829939| Beliaev and Saffarini 1998]) | (LML recipes from [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829939| Beliaev and Saffarini 1998]) | ||
==Chamber media== | |||
* 5.844 g/L 100mM NaCl | |||
* 15.1185 g/L 50mM PIPES (hydrogen) | |||
''7.0 pH'' | |||
==Phosphate buffer== | |||
* 2.918 g/L Monosodium phosphate, monohydrate | |||
* 0.7733 g/L Disodium phosphate, heptahydrate | |||
''7.0 pH'' | |||
Revision as of 09:24, 11 July 2008
Recipes
LML medium (for anaerobic Shewanella growth)
- 0.02% yeast extract
- 0.01% peptone
- 20 mM lactate
- 10 mM HEPES [pH 7.4]
LML agar (for Shewanella reduction test)
- LML medium
- 0.8% agar
- 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))
(LML recipes from Beliaev and Saffarini 1998)
Chamber media
- 5.844 g/L 100mM NaCl
- 15.1185 g/L 50mM PIPES (hydrogen)
7.0 pH
Phosphate buffer
- 2.918 g/L Monosodium phosphate, monohydrate
- 0.7733 g/L Disodium phosphate, heptahydrate
7.0 pH
