IGEM:Harvard/2007/Plates
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Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07
| Cell Type | Volume Plated | Colony Count (Short Ligation) |
Colony Count (Overnight Ligation) |
| OmpA1 + random library, PCR | 275 µL | 5 | 41 |
| OmpA1 + random library, PCR | 25 µL | 0 | 2 |
| OmpA1 + random library, extension | 275 µL | 0 | 30 |
| OmpA1 + random library, extension | 25 µL | 0 | 0 |
| OmpA2 + random library, PCR | 275 µL | 0 | 1 |
| OmpA2 + random library, PCR | 25 µL | 0 | 0 |
| OmpA2 + random library, extension | 275 µL | 12 | 77 |
| OmpA2 + random library, extension | 25 µL | 0 | 1 |
Plated 6/25/07
The following were all done with 275 uL:
| Cell Type | Colony Count (Overweekend Ligation;1uL DNA) |
Colony Count (Overweekend Ligation;6uL DNA) |
| OmpA1/Group1/S | 0 | 127 |
| OmpA1/Group1/S2 | 8 | 24 |
| OmpA1/Group2/B | 10 | 119 |
| OmpA1/Group2/S2 | 4 | 79 |
