IGEM:Harvard/2007/Kevin's 91r Notebook: Difference between revisions
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(New page: *Step 1: Overnight culture from Perry's frozen stock of bacteria with LppOmpA plasmid. *Step 2: Plasmid prep of LppOmpA plasmid. *Step 3: Purification of plasmid. *Step 4: Design primers ...) |
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Revision as of 13:32, 26 November 2007
- Step 1: Overnight culture from Perry's frozen stock of bacteria with LppOmpA plasmid.
- Step 2: Plasmid prep of LppOmpA plasmid.
- Step 3: Purification of plasmid.
- Step 4: Design primers for PCR.
- Step 5: Produce PCR product and run on gel.
- Step 6: Topo cloning reaction: Mix PCR product and pTrcHis2-TOPO
- Step 7: Transform mix into OneShot E.coli cells
- Step 8: Overnight culture of colonies to analyze positive clones
- Step 9: Plasmid prep of culture
- Step 10: Digestion with Nco1 and EcoR1 and run on gel.
