IGEM:Harvard/2007/Gels: Difference between revisions
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[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]] | == Week 0 gel == | ||
[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]]<br> | |||
Description: Week 0 gel<br> | |||
Description: Week 0 gel | |||
Well 1 Ladder <br> | Well 1 Ladder <br> | ||
Well 2 OmpA1 in PET <br> | Well 2 OmpA1 in PET <br> | ||
Line 9: | Line 9: | ||
Well 5 OmpA1 +strep tag in PET <br> | Well 5 OmpA1 +strep tag in PET <br> | ||
Well 6 OmpA2 +his tag in PET <br> | Well 6 OmpA2 +his tag in PET <br> | ||
Well 7 OmpA2 +strep tag in PET <br> | Well 7 OmpA2 +strep tag in PET <br><br><br><br> | ||
[[Image:June 19 Gel.jpg|thumb|left|250px]] | == 6/20 gel == | ||
[[Image:June 19 Gel.jpg|thumb|left|250px]] | |||
Description: 6/20 gel<br> | Description: 6/20 gel<br> | ||
Well 1 Ladder (10 kb) | Well 1 Ladder (10 kb) <br> | ||
Well 2 OmpA1, Nhe1, Pst1, Group 1 27.5 uL<br> | |||
Well 2 OmpA1, Nhe1, Pst1, Group 1 <br> | Well 3 OmpA1, Nhe1, Pst1, Group 1 27.5 uL<br> | ||
Well 3 OmpA1, Nhe1, Pst1, Group 1 <br> | Well 4 OmpA1, Nhe1, Pst1, Group 2 27.5 uL<br> | ||
Well 4 OmpA1, Nhe1, Pst1, Group 2 <br> | Well 5 OmpA1, Nhe1, Pst1, Group 2 27.5 uL<br> | ||
Well 5 OmpA1, Nhe1, Pst1, Group 2 <br> | Well 6 OmpA1, Nhe1, Pst1, Group 2 27.5 uL<br> | ||
Well 6 OmpA1, Nhe1, Pst1, Group 2 <br> | Well 7 OmpA2, Nhe1, Pst1, Group 4 27.5 uL<br> | ||
Well 7 OmpA2, Nhe1, Pst1, Group 4 <br> | Well 8 OmpA2, Nhe1, Pst1, Group 4 27.5 uL<br> | ||
Well 8 OmpA2, Nhe1, Pst1, Group 4 <br> | Well 9 OmpA2, Nhe1, Pst1, Group 3 27.5 uL<br> | ||
Well 9 OmpA2, Nhe1, Pst1, Group 3 <br> | Well 10 OmpA2, Nhe1, Pst1, Perry's 27.5 uL<br> | ||
Well 10 OmpA2, Nhe1, Pst1, Perry's<br> | |||
Well 11 OmpA2, Nhe1, Pst1, Perry's<br> | Well 11 OmpA2, Nhe1, Pst1, Perry's<br> | ||
See Notebook page for gel extraction weights and values.<br> | See Notebook page for gel extraction weights and values.<br> | ||
== 6/19 gel after excision== | |||
[[Image:6-19-07_gel_after_excision.jpg|thumb|left|250px]]<br> | |||
[[Image:6-19-07_gel_after_excision.jpg|thumb|left|250px]] | |||
Description: 6/19 gel after excision <br> | Description: 6/19 gel after excision <br> | ||
Line 47: | Line 39: | ||
Well 5 OmpA1 +strep tag in PET <br> | Well 5 OmpA1 +strep tag in PET <br> | ||
Well 6 OmpA2 +his tag in PET <br> | Well 6 OmpA2 +his tag in PET <br> | ||
Well 7 OmpA2 +strep tag in PET <br> | Well 7 OmpA2 +strep tag in PET <br><br><br><br> | ||
== 6/22 overexpression gel == | |||
[[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]] | [[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]] | ||
Description: 6/22 overexpression gel <br> | <br>Description: 6/22 overexpression gel <br> | ||
lane 1 Ladder <br> | lane 1 Ladder <br> | ||
lane 2 OmpA1 + his6 non-induced<br> | lane 2 OmpA1 + his6 non-induced<br> | ||
Line 66: | Line 53: | ||
lane 7 OmpA2 + his6 induced <br> | lane 7 OmpA2 + his6 induced <br> | ||
lane 8 OmpA2 + strep2 non-induced <br> | lane 8 OmpA2 + strep2 non-induced <br> | ||
lane 9 OmpA2 + strep2 induced <br> | lane 9 OmpA2 + strep2 induced <br><br> | ||
== 6/22 overexpression gel2, ladder contaminated == | |||
[[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]] | [[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]] | ||
<br><br><br> | |||
Description: Contaminated 6/22 overexpression gel <br> | Description: Contaminated 6/22 overexpression gel <br> | ||
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added. | Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added. |
Revision as of 10:33, 27 June 2007
Week 0 gel
Description: Week 0 gel
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
6/20 gel
Description: 6/20 gel
Well 1 Ladder (10 kb)
Well 2 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 3 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 4 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 5 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 6 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 7 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 8 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 9 OmpA2, Nhe1, Pst1, Group 3 27.5 uL
Well 10 OmpA2, Nhe1, Pst1, Perry's 27.5 uL
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.
6/19 gel after excision
Description: 6/19 gel after excision
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
6/22 overexpression gel
Description: 6/22 overexpression gel
lane 1 Ladder
lane 2 OmpA1 + his6 non-induced
lane 3 OmpA1 + his6 induced
lane 4 OmpA1 + strep2 non-induced
lane 5 OmpA1 + strep2 induced
lane 6 OmpA2 + his6 non-induced
lane 7 OmpA2 + his6 induced
lane 8 OmpA2 + strep2 non-induced
lane 9 OmpA2 + strep2 induced
6/22 overexpression gel2, ladder contaminated
Description: Contaminated 6/22 overexpression gel
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.