IGEM:Harvard/2007/Gels: Difference between revisions
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[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]] | [[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]] | ||
Description: Week 0 gel | |||
Well 1 Ladder <br> | Well 1 Ladder <br> | ||
Well 2 OmpA1 in PET <br> | Well 2 OmpA1 in PET <br> | ||
| Line 10: | Line 10: | ||
Well 6 OmpA2 +his tag in PET <br> | Well 6 OmpA2 +his tag in PET <br> | ||
Well 7 OmpA2 +strep tag in PET <br> | Well 7 OmpA2 +strep tag in PET <br> | ||
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[[Image:June 19 Gel.jpg|thumb|left|250px]]<br> | [[Image:June 19 Gel.jpg|thumb|left|250px]]<br> | ||
Description: 6/20 gel<br> | |||
Well 1 Ladder (10 kb) | Well 1 Ladder (10 kb) | ||
<br> 27.5 uL each of: <br> | <br> 27.5 uL each of: <br> | ||
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Description: 6/19 gel after excision <br> | |||
Well 1 Ladder <br> | Well 1 Ladder <br> | ||
Well 2 OmpA1 in PET <br> | Well 2 OmpA1 in PET <br> | ||
| Line 53: | Line 56: | ||
[[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]] | [[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]] | ||
Description: 6/22 overexpression gel <br> | |||
lane 1 Ladder <br> | lane 1 Ladder <br> | ||
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[[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]] | [[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]] | ||
Description: Contaminated 6/22 overexpression gel <br> | |||
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added. | Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added. | ||
Revision as of 09:17, 22 June 2007
Description: Week 0 gel
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
Description: 6/20 gel
Well 1 Ladder (10 kb)
27.5 uL each of:
Well 2 OmpA1, Nhe1, Pst1, Group 1
Well 3 OmpA1, Nhe1, Pst1, Group 1
Well 4 OmpA1, Nhe1, Pst1, Group 2
Well 5 OmpA1, Nhe1, Pst1, Group 2
Well 6 OmpA1, Nhe1, Pst1, Group 2
Well 7 OmpA2, Nhe1, Pst1, Group 4
Well 8 OmpA2, Nhe1, Pst1, Group 4
Well 9 OmpA2, Nhe1, Pst1, Group 3
Well 10 OmpA2, Nhe1, Pst1, Perry's
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.
Description: 6/19 gel after excision
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
Description: 6/22 overexpression gel
lane 1 Ladder
lane 2 OmpA2 & strep non-induced
lane 3 OmpA2 & his induced immediately
lane 4 OmpA1 & strep induced immediately
lane 5 OmpA1 & his induced immediately PET
lane 6 OmpA2 & strep non-induced
lane 7 OmpA2 & his non-induced
Description: Contaminated 6/22 overexpression gel
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.
