IGEM:Harvard/2007/Brainstorming/

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m (Adhesion Targets)
m (Adhesion Targets)
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'''Mammalian cell surface proteins'''
'''Mammalian cell surface proteins'''
-
*gp75 - Surface expression of gp75 on mouse melanoma cells correlates with the ability of a monoclonal antibody against gp75 to reject melanomas in syngeneic mice
+
 
'''Protozoan membrane proteins'''
'''Protozoan membrane proteins'''
-
*By the way, I was thinking about expressing plasmodium falciparum proteins on the surface of E coli and maybe showing somehow that an immune rxn can be elicited in mice or sthing like that e.g.  in humans, "High levels of plasma chitotriosidase are a marker of macrophage activation..."
+
*By the way, I was thinking about expressing plasmodium falciparum proteins on the surface of E coli and maybe showing somehow that an immune rxn can be elicited in mice or something like that e.g.  in humans, "High levels of plasma chitotriosidase are a marker of macrophage activation..."
*Speaking on targets, heregoes:
*Speaking on targets, heregoes:
*MSP-1 --> well characterized
*MSP-1 --> well characterized
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'''Viral Membrane Proteins'''
'''Viral Membrane Proteins'''
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*Influenza A M2 Protein
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*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3882238&dopt=Abstract Influenza A M2 Protein]
*Neuraminidase
*Neuraminidase
*Hemaglutinin
*Hemaglutinin
*Semliki Forest membrane proteins
*Semliki Forest membrane proteins
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*Epstein Barr LMP1/LMP2
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*[http://jvi.asm.org/cgi/content/full/79/7/4415 Epstein Barr LMP1]
 +
*[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=249393 Epstein Barr LMP2]
*HIV gp120
*HIV gp120
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'''Cancer Membrane Proteins'''
+
'''Cancer-Related Membrane Proteins'''
*[http://www.molecular-cancer.com/content/5/1/41 Eag1 Potassium Channels]
*[http://www.molecular-cancer.com/content/5/1/41 Eag1 Potassium Channels]
*[http://bioinformatics.org/pcgdb/Genes/muc1/muc1.htm Muc1]
*[http://bioinformatics.org/pcgdb/Genes/muc1/muc1.htm Muc1]
-
*NGEP-L
+
*[http://cancerres.aacrjournals.org/cgi/content/abstract/67/4/1594 NGEP-L]
*[http://clincancerres.aacrjournals.org/cgi/content/abstract/7/12/4182?ck=nck HP59]
*[http://clincancerres.aacrjournals.org/cgi/content/abstract/7/12/4182?ck=nck HP59]
 +
*[http://clincancerres.aacrjournals.org/cgi/content/abstract/2/11/1837 gp75] - Surface expression of gp75 on mouse melanoma cells correlates with the ability of a monoclonal antibody against gp75 to reject melanomas in syngeneic mice
 +

Revision as of 00:18, 16 May 2007

Contents

Adhesion Targets

Brainstorming of Possible Adhesion Targets (feel free to add/reorganize/whatever--I just thought I'd get this started):

  • Keratins -- was thinking of new lines of "hair conditioners" --> these tend to add body to the hair shaft by using bonding polymers; I was thinking that bacteria could be the new line of "bonding polyproteinecious entities" etc etc


textiles

  • silk
  • wool
  • polyester
  • nylon fibres
  • Could use the MIT idea to clone E coli that secrete pleasant smells and similarly bind to these fibers -- a living deodorant!


eukaryotic cells

  • Mosquito gut epithelial cells as targets


other mammalian

  • Laminins
  • fibronectin


Mammalian cell surface proteins


Protozoan membrane proteins

  • By the way, I was thinking about expressing plasmodium falciparum proteins on the surface of E coli and maybe showing somehow that an immune rxn can be elicited in mice or something like that e.g. in humans, "High levels of plasma chitotriosidase are a marker of macrophage activation..."
  • Speaking on targets, heregoes:
  • MSP-1 --> well characterized
  • CSP
  • EBA-175, a 175 kDa 'erythrocyte binding antigen' from P. falciparum
  • DBP, Duffy-binding protein from P. vivax and P. knowlesi
  • SSP2, Plasmodium sporozoite surface protein-2. Also known as TRAP (thrombospondin-related adhesive protein).
  • Proteins with homology to SSP2/TRAP from Toxoplasma (MIC2), Eimeria (Etp100), and Cryptosporidium
  • CTRP, circumsporozoite- and TRAP-related protein of Plasmodium found in the ookinete stage
  • (see http://www.tulane.edu/~wiser/malaria/cmb.html#junction)
  • MSP-1 polypeptide fragments


Viral Membrane Proteins


Cancer-Related Membrane Proteins


Assorted

  • Presenilin

2 Component Systems

  • We should start researching these as well as the tagets.
  • Perhaps The TF's (myself included, of course) can help point out some papers with which to start.

- Nick

  • How about arsenic sensor using an ars regulator, ars promoter and GFP gene -- we can have it just warn one if the arsenical concentration is greater than 5 ppb.
  • Maybe look over the light sensitive bacteria paper again, they use a 2 component system there.
  1. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, and Voigt CA. . pmid:16306980. PubMed HubMed [sb2]



Cascade Network Structure

  • Some loose brainstorming on a possible reporter network that can be activated from the membrane protein as we discussed would be beneficial as well, if we plan to do something like that.
  • May be good to start with something similar to what has been done previously and hook it up to GFP.
  • Some TF direction would be warranted here as well.

- Nick


Previous Brainstorming Ideas

Btw, can we reorganize the brainstorming page to put the older brainstorming into folders or something and only have the stuff relevant to our project still on the front page? I have no idea how to do this...thanks. -Shaunak

Hope you like the formatting.
--Stephanie (5/12)

Brainstorming, 4/23 to 5/9
Misc Brainstorming, pre-5/9
Brainstorming from Second Meeting, 4/05
Brainstorming from First Meeting, 3/19



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