IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-30: Difference between revisions
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8% PEG | ===8% PEG=== | ||
- 20 {{ul}} 20% PEG + 5 {{ul}} nanostructures + 5 {{ul}} NaCl | - 20 {{ul}} 20% PEG + 5 {{ul}} nanostructures + 5 {{ul}} NaCl | ||
- put on ice 15 min. | - put on ice 15 min. | ||
- spin @ 16k rcf for 10 min. | - spin @ 16k rcf for 10 min. | ||
- pipette out supernatant | - pipette out supernatant | ||
- resuspend pellet in 10x folding buffer | - resuspend pellet in 10x folding buffer | ||
Latest revision as of 13:07, 5 December 2006
8% PEG
- 20 μL 20% PEG + 5 μL nanostructures + 5 μL NaCl
- put on ice 15 min.
- spin @ 16k rcf for 10 min.
- pipette out supernatant
- resuspend pellet in 10x folding buffer
