IGEM:Harvard/2006/DNA nanostructures: Difference between revisions

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<div class="tabs-blue">
<ul>
<li id="current">[[IGEM:Harvard/2006/DNA nanostructures|Project Overview]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Designs|Designs]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Notebook|Notebook]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Protocols|Protocols]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Presentations|Presentations]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Literature|Literature]]</li>
</ul>
</div>
<br style="clear:both">
==Project Overview==
==Project Overview==
*Our goal is to to design and implement molecular containers, which can be dynamically opened and closed by an external stimulus.
*Our goal is to design and implement molecular containers, which can be dynamically opened and closed by an external stimulus.
*The containers will be implemented as DNA nanostructures, which afford a significant degree of positional control and chemical versatility.
*The containers will be implemented as DNA nanostructures, which afford a significant degree of positional control and chemical versatility.
*As an initial proof-of-concept, we plan to use our DNA containers to demonstrate controllable activation ("delivery") of anti-thrombin aptamers.
*As an initial proof-of-concept, we plan to use our DNA containers to demonstrate controllable activation ("delivery") of anti-thrombin aptamers.
Line 12: Line 25:
**Cell sorting
**Cell sorting


==Container Specs==
==Working Team Members==
[[Image:iGEM_harv06_mattspecs.gif]]
*[[User:TChan|Tiffany Chan]] ([[User_talk:TChan|talk]], [[Special:Contributions/TChan|edits]])
 
*[[User:Kfifer|Katherine Fifer]] ([[User_talk:Kfifer|talk]], [[Special:Contributions/Kfifer|edits]])
==Container Designs==
*[[User:Vlau|Valerie Lau]] ([[User_talk:Vlau|talk]], [[Special:Contributions/Vlau|edits]])
<gallery>
*[[User:Matthewmeisel|Matthew Meisel]] ([[User_talk:Matthewmeisel|talk]], [[Special:Contributions/Matthewmeisel|edits]])
Image:Igemharv06_Katie_Val_cylinderI.gif|[[IGEM:Harvard/2006/Container Design 1|Design 1]]<br>hexagonal core, separate 1-ply lids
*[[User:Lhahn|Lewis Hahn]] ([[User_talk:Lhahn|talk]], [[Special:Contributions/Lhahn|edits]])
Image:Smallcontainerdesign2.jpg|[[IGEM:Harvard/2006/Container Design 2|Design 2]]<br>hexagonal core, separate 2-ply lids
*TA: [[User:ShawnDouglas|Shawn Douglas]] ([[User_talk:ShawnDouglas|talk]], [[Special:Contributions/ShawnDouglas|edits]])
Image:Igemharv06_msmrect.png|[[IGEM:Harvard/2006/Container Design 3|Design 3]]<br>rectangular core, continuous 1-ply lids
Image:Websmallbarrsingleply.jpg|[[IGEM:Harvard/2006/Container Design 4|Design 4]]<br>hexagonal core, separate 1-ply lids
</gallery>
 
==Latch Designs==
<gallery>
Image:iGEM_harv06_mattlatch1.jpg |latch1 <br>[[:Media:iGEM_harv06_mattlatch1.jpg|jpg]] | [[:Media:IGEM_harv06_mattlatch1.ai|ai]]
Image:iGEM_harv06_mattlatch2.jpg |latch2 <br>[[:Media:iGEM_harv06_mattlatch2.jpg|jpg]] | [[:Media:IGEM_harv06_mattlatch2.ai|ai]]
</gallery>
 
==Coding==
===Existing code===
*[[IGEM:Harvard/2006/DNA_nanostructures/Designing_DNA_nanostructures|William's code (Python)]]
 
==Thrombin-aptamer experiments==


====Notes====
==Recent Changes==
 
{{Special:Recentchanges/b=IGEM:Harvard/2006/DNA_nanostructures/&limit=20}}
Questions
* what percent gel? 10% to 20% polyacrylamide gels, no SDS (but would make for a good control)
* what incubation conditions?
* how much protein and DNA? protein at 1 {{um}}, DNA at 2 {{um}}
* DNA in excess
* experiments
** all combinations protein, aptamer, nanotube
 
Buffers
* Macaya's and Bock's selection buffer: 20 mM Tris-acetate, pH 7.4, 140 mM NaCl / 5 mM KCl / 1 mM CaCl<sub>2</sub> / 1 mM MgCl<sub>2</sub>
 
====Bibliography====
<biblio>
# tha1 pmid=8107090
# tha2 pmid=15945116
# tha3 pmid=8298130
# tha4 pmid=1741036
# tha5 pmid=8475124
</biblio>
 
==Presentations==
 
===Most recent (Week 3)===
* [[Media:IGEMHarv06 Week3 presentation VKTM2.ppt|Week 3 Presentation: Design Progress]]
 
===Week 2: Original proposal===
* [[IGEM:Harvard/2006/DNA_nanostructures/Presentation_proposal|Presentation Proposal]]
 
==Working Team Members==
*[[User:TChan|Tiffany Chan]] ([[User_talk:TChan|talk]])
*[[User:Kfifer|Katherine Fifer]] ([[User_talk:Kfifer|talk]])
*[[User:Vlau|Valerie Lau]] ([[User_talk:Vlau|talk]])
*[[User:Matthewmeisel|Matthew Meisel]] ([[User_talk:Matthewmeisel|talk]])
*...and others are welcome!

Latest revision as of 18:15, 28 October 2006



Project Overview

  • Our goal is to design and implement molecular containers, which can be dynamically opened and closed by an external stimulus.
  • The containers will be implemented as DNA nanostructures, which afford a significant degree of positional control and chemical versatility.
  • As an initial proof-of-concept, we plan to use our DNA containers to demonstrate controllable activation ("delivery") of anti-thrombin aptamers.
  • We expect that molecular containers could have several interesting scientific and clinical applications, such as
    • Drug and gene delivery
    • Bio-marker scavenging (early detection of biomarkers)
    • Directed evolution (compartmentalized selections)
    • Using multiplexing for combinatorial chemical synthesis
    • Capture and stabilization of multiprotein complexes
    • Protein folding (chaperones)
    • Cell sorting

Working Team Members

Recent Changes

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17 April 2024

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