IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29

Midiprep of J036335 (KaiA+KaiC), J036832 (KaiB)

Midiprep of KaiA+KaiC, KaiB was successful.

Digest of J036335\ES (KaiA + KaiC), J036832\EX (KaiB)

KaiA+KaiC \ES: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Spe1 (3.4)
         * 0.2 µL Pst1 (3.4) 

KaiB \ EX: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Xba1 (3.4)
         * 0.2 µL Pst1 (3.4)

This digest failed for some reason (EcoRI + SpeI should use NEBuffer EcoRI, not NEBuffer 2)

Digest of J036335\SP (KaiA + KaiC), J036832\XP (KaiB)

9 samples each.

Jeff to-do

At 10PM:

• CIP the A+C\S-P for 1h
• Run a gel (Ladder, undigested, digest...)