IGEM:Harvard/2006/Brainstorming Papers - Jeffrey
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Self organization
- Basu S, Gerchman Y, Collins CH, Arnold FH, and Weiss R. A synthetic multicellular system for programmed pattern formation. Nature. 2005 Apr 28;434(7037):1130-4. DOI:10.1038/nature03461 |
- Basu S, Mehreja R, Thiberge S, Chen MT, and Weiss R. Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6355-60. DOI:10.1073/pnas.0307571101 |
A synthetic multicellular system for programmed pattern formation.
- Basu et al created a gene circuit with a non-monotonic response to AHL concentration.
- Main idea: use two concentration boundaries to define three regions with different transcriptional activity
- GFP is repressed at low and high concentrations of AHL, and expressed at medium concentrations
- Basu et al. created three variants with different upper boundaries
- Concentration varies with distance; thus, a mixture of different variants of bacteria produces a bullseye pattern when exposed to a point source of AHL
- Multiple AHL sources produce pretty pictures
- We could reuse the basic idea behind their circuit if we need to define a multiple-region response to some stimulus