IGEM:Harvard/2006/Brainstorming Papers - Jeffrey: Difference between revisions
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'''Spatiotemporal control of gene expression with pulse-generating networks''' | '''Spatiotemporal control of gene expression with pulse-generating networks''' | ||
*Basu ''et al.'' created a gene circuit that exhibits transient expression of GFP in response to AHL. | *Basu ''et al.'' created a gene circuit that exhibits transient expression of GFP in response to AHL ("pulse generator"). | ||
*The amplitude and duration of this "pulse" can be artificially adjusted, and depend on the rate of increase of AHL concentration as well as the absolute concentration. | *The amplitude and duration of this "pulse" can be artificially adjusted, and depend on the rate of increase of AHL concentration as well as the absolute concentration. | ||
**Cells can be made to respond to nearby cells and ignore far-away cells | **Cells can be made to respond to nearby cells and ignore far-away cells | ||
***Reporter expression varies with distance, even though AHL concentrations eventually reach the same level everywhere | |||
*Further tweaking: | |||
**Produce AHL as well as expressing GFP, to propagate the AHL signal | |||
**Connect the output of the pulse generator to a bistable switch |
Latest revision as of 12:09, 16 June 2006
Self organization
- Basu S, Gerchman Y, Collins CH, Arnold FH, and Weiss R. A synthetic multicellular system for programmed pattern formation. Nature. 2005 Apr 28;434(7037):1130-4. DOI:10.1038/nature03461 |
- Basu S, Mehreja R, Thiberge S, Chen MT, and Weiss R. Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6355-60. DOI:10.1073/pnas.0307571101 |
A synthetic multicellular system for programmed pattern formation.
- Basu et al. created a gene circuit with a non-monotonic response to AHL concentration.
- Main idea: use two concentration boundaries to define three regions with different transcriptional activity
- GFP is repressed at low and high concentrations of AHL, and expressed at medium concentrations
- Basu et al. created three variants with different upper boundaries
- Concentration varies with distance; thus, a mixture of different variants of bacteria produces a bullseye pattern when exposed to a point source of AHL
- Multiple AHL sources produce pretty pictures
- We could reuse the basic idea behind their circuit if we need to define a multiple-region response to some stimulus
Spatiotemporal control of gene expression with pulse-generating networks
- Basu et al. created a gene circuit that exhibits transient expression of GFP in response to AHL ("pulse generator").
- The amplitude and duration of this "pulse" can be artificially adjusted, and depend on the rate of increase of AHL concentration as well as the absolute concentration.
- Cells can be made to respond to nearby cells and ignore far-away cells
- Reporter expression varies with distance, even though AHL concentrations eventually reach the same level everywhere
- Cells can be made to respond to nearby cells and ignore far-away cells
- Further tweaking:
- Produce AHL as well as expressing GFP, to propagate the AHL signal
- Connect the output of the pulse generator to a bistable switch