IGEM:Harvard/2006/Brainstorming Papers - Hetmann

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Cyanobacteria Oscillators

  1. Wang J. . pmid:15893664. PubMed HubMed [1]
  2. Nakajima M, Imai K, Ito H, Nishiwaki T, Murayama Y, Iwasaki H, Oyama T, and Kondo T. . pmid:15831759. PubMed HubMed [2]
  3. Imai K, Nishiwaki T, Kondo T, and Iwasaki H. . pmid:15229218. PubMed HubMed [3]
  4. Naef F. . pmid:16729054. PubMed HubMed [4]
All Medline abstracts: PubMed HubMed

[Full text article of Naef]

[Full text article of Wang J.]

Nakajima et. al

  • cyanobacterium Synechococcus elongatus (PCC 7942)
  • three genes components (kaiA, kaiB, and kaiC)
  • Kai proteins regulate the kaiBC operon
    • Oscillation of KaiC phosphorylation cycle is the pacemaker, molecular timer
    • KaiA enhances KaiC autophosphorylation
    • KaiB attenuates the effect of KaiA
    • Oscillation of KaiC phosphorylation maybe generated by KaiA and KaiB cooperation
  • Nakajima et. al incubated recombinant KaiC protein with KaiA and KaiB at a ratio similar to in vivo to test it in vitro
  • KaiC phosphorylation oscillated robustly without damping
  • KaiC not degraded during the reaction
  • Oscillation of KaiC phosphorylation is autonomous due to cooperation of three Kai proteins
  • The thermal sensitivity in vitro same as in vivo gene expression rhythm
  • They found that KaiC phosphorylation is the molecular timer for the circadian rhythm of Synechococcus
  • KaiC hexamer may have many phosphorylation states with different biochemical characteristics
  • In addition, the reaction rate of KaiC phosphorylation and dephosphorylation are slow reducing the energy needed

[Nakajima et. al article]

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