All these vectors were kindly provided by Dr. Dan Ziegler, Director of the Bacillus Genetic Stock Center at Ohio State University.
Daniel Goodman 17:01, 31 July 2008 (UTC):I just put this table together, but I have yet to add the plasmid maps to these. For now, to see the maps, go to BGSC homepage and find them in the catalog. They are also in the black Turing Group binder under "ordered vectors and strains".
Integration Vectors
These vectors integrate into the chromosome and do not have a replication origin in Bacillus. They either integrate cassettes that require double crossovers and two homologous regions, or whole-plasmid insertions that only require one crossover and one corresponding region of homology.
Ectopic Integration
The integration site of these is pre-determined
BGSC Accession
|
Vector Name
|
Features
|
Integration Locus
|
Vector Map
|
Vector Sequence
|
ECE112
|
pDG1661
|
- Chloramphenicol selectable in EC and BS
- Spectinomycin selectable only in EC
- spoVG-lacZ fusion: contains spoVG RBS
- insert must contain a promoter
- cassette insertion
|
amyE
|
map
|
U46196
|
ECE171
|
pPyr-Kan
|
- Kanamycin selectable in EC or BS
- Ampicillin selectable only in EC
- cassette insertion
|
pyrD
|
map
|
AY464559
|
ECE172
|
pGlt-Cm
|
- Cloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- cassette insertion
|
gltA
|
map
|
AY464560
|
ECE176
|
pBCJ164
|
- Cloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- cassette insertion
- contains strong rspD promoter and RBS
|
rspD
|
map
|
BGSC sequence
|
ECE153
|
pSG1154
|
- Spectinomycin selectable in EC or BS
- Ampicillin selectable only in EC
- cassette insertion
- xylose-inducible promoter
- high fluorescence GFP variant for protein fusions
|
amyE
|
map
|
not available
|
ECE162
|
pSG1193
|
- Spectinomycin selectable in EC or BS
- Ampicillin selectable only in EC
- YFP full coding sequence, including RBS and Pxyl
- xylose-inducible
- cassette insertion
|
amyE
|
map
|
not available
|
Insert Integration
The user inserts a homologous piece of the Bacillus chromosome and the vector integrates there.
BGSC Accession
|
Vector Name
|
Features
|
Vector Map
|
Vector Sequence
|
ECE149
|
pMUTIN-GFP+
|
- Erythromycin selectable in EC or BS
- Ampicillin selectable only in EC
- GFP+ full coding sequence, including RBS and Pspac
- IPTG-inducible
- full plasmid insertion
|
map
|
From Bayreuth
|
ECE150
|
pMUTIN-CFP
|
- Erythromycin selectable in EC or BS
- Ampicillin selectable only in EC
- CFP full coding sequence, including RBS and Pspac
- IPTG-inducible
- full plasmid insertion
|
map
|
From Bayreuth
|
ECE151
|
pMUTIN-YFP
|
- Erythromycin selectable in EC or BS
- Ampicillin selectable only in EC
- YFP full coding sequence, including RBS and Pspac
- IPTG-inducible
- full plasmid insertion
|
map
|
From Bayreuth
|
ECE147
|
pMUTIN-cMyc
|
- Erythromycin selectable in EC or BS
- Ampicillin selectable only in EC
- cMyc antibody tag fusion
- IPTG-inducible Pspac promoter
|
map
|
From Bayreuth
|
Shuttle Vectors
BGSC Accession
|
Vector Name
|
Features
|
Vector Map
|
Vector Sequence
|
ECE165
|
pAD123
|
- Chloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- gfpmut3a in front of MCS, no promoter, but gram-positive RBS
|
map
|
BGSC sequence
|
ECE166
|
pAD43-25
|
- Chloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- gfpmut3a in front of MCS, strong Pupp promoter, gram-positive RBS
|
map
|
BGSC sequence
|
ECE188
|
pHCMC02
|
- Chloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- weak constitutive PlepA promoter in front of MCS
|
map
|
From Bayreuth
|
ECE189
|
pHCMC04
|
- Chloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- strong xylose inducible PxylA promoter in front of MCS
|
map
|
From Bayreuth
|
ECE190
|
pHCMC05
|
- Chloramphenicol selectable in EC or BS
- Ampicillin selectable only in EC
- strong xylose inducible PxylA promoter in front of MCS
|
map
|
From Bayreuth
|