IGEM:Cambridge/2008/Turing Pattern Formation/Vectors: Difference between revisions
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(New page: These vectors integrate into the chromosome and do not have a replication origin in Bacillus. They either integrate cassettes that require double crossovers and two homologous regions, or ...) |
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Revision as of 09:57, 31 July 2008
These vectors integrate into the chromosome and do not have a replication origin in Bacillus. They either integrate cassettes that require double crossovers and two homologous regions, or whole-plasmid insertions that only require one crossover and one corresponding region of homology.
Ectopic Integration
The integration site of these is pre-determined
BGSC Accession | Vector Name | Features | Integration Locus | Vector Map | Vector Sequence |
---|---|---|---|---|---|
ECE112 | pDG1661 |
|
amyE | map | U46196 |
ECE171 | pPyr-Kan |
|
pyrD | map | AY464559 |
ECE172 | pGlt-Cm |
|
gltA | map | AY464560 |
ECE176 | pBCJ164 |
|
rspD | map | BGSC sequence |
ECE153 | pSG1154 |
|
amyE | map | not available |
ECE162 | pSG1193 |
|
amyE | map | not available |
Insert Integration
The user inserts a homologous piece of the Bacillus chromosome and the vector integrates there.
BGSC Accession | Vector Name | Features | Vector Map | Vector Sequence |
---|---|---|---|---|
ECE149 | pMUTIN-GFP+ |
|
map | From Bayreuth |
ECE150 | pMUTIN-CFP |
|
map | From Bayreuth |
ECE151 | pMUTIN-YFP |
|
map | From Bayreuth |
ECE147 | pMUTIN-cMyc |
|
map | From Bayreuth |
Shuttle Vectors
BGSC Accession | Vector Name | Features | Vector Map | Vector Sequence |
---|---|---|---|---|
ECE165 | pAD123 |
|
map | BGSC sequence |
ECE166 | pAD43-25 |
|
map | BGSC sequence |
ECE188 | pHCMC02 |
|
map | [1] |
ECE189 | pHCMC04 |
|
map | [2] |
ECE190 | pHCMC05 |
|
map | [3] |