IGEM:Cambridge/2008/Turing Pattern Formation/Primers

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Revision as of 04:09, 5 August 2008 by Daniel Goodman (talk | contribs) (New page: ====Bacillus RBS Primers==== We made primer sets for 2 ribosomal binding sites in Bacillus of differing binding strengths. We expect B._sub_RBSs to bind very strongly because it is the co...)
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Bacillus RBS Primers

We made primer sets for 2 ribosomal binding sites in Bacillus of differing binding strengths. We expect B._sub_RBSs to bind very strongly because it is the consensus sequence for RBS in B. subtilis.


B. subtilis consensus RBS - http://www.ncbi.nlm.nih.gov/pubmed/10446248

B._sub_RBSs_F

  • 5' GAATTCGCGGCCGCTTCTAGAG AAAGGAGG TGTTA 3'

B._sub_RBSs_R

  • 5' CTGCAGCGGCCGCTACTAGTAACA CCTCCTTT CTCT 3'


RBS modified from ECE112 SpoVG RBS - http://www.bgsc.org/Catalogs/Catpart4.pdf

B._sub_RBSw_F

  • 5' GAATTCGCGGCCGCTTCTAGAG AGAGGTGG TGTTA 3'

B._sub_RBSw_R

  • 5' CTGCAGCGGCCGCTACTAGTAACA CCACCTCT CTCT 3'


Agr Primers

  • These primers are to extract each part of the Agr operon so that a Bacillus RBS can replace the E. coli RBS currently attached to the genes.
agr senders

Agr_B_F

  • 5' GTTTCTT C GAATTC GCGGCCGC T TCTAG ATGaactattttgacaacaa 3'

Agr_B_R

  • 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA tttcagatcctctttgatg 3'

Agr_D_F

  • 5' CTT C GAATTC GCGGCCGC T TCTAG atgaatactctgttcaatctgtttt 3'

Agr_D_R

  • 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA ttcatgcagctgggtcagct 3'
agr receivers

Agr_C_F

  • 5' GTTTCTT C GAATTC GCGGCCGC T TCTAG atgattctgatgttcaccat 3'

Agr_C_R

  • 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA gttattgatgatttcgactt 3'

Agr_A_F

  • 5' GTTTCTT C GAATTC GCGGCCGC T TCTAG atggaaatcgcactggcta 3'

Agr_A_R

  • 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA gattttcttgacattgcgta 3'
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