IGEM:Cambridge/2008/Turing Pattern Formation/Experiments: Difference between revisions

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(New page: ===Finding and Biobricking proper Ribosomal Binding Sites=== *[http://partsregistry.org/Assembly:RBS-CDS_issues Solving Biobrick RBS issues] ===Testing Sender and Reciever=== *We can t...)
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Revision as of 07:35, 31 July 2008

Finding and Biobricking proper Ribosomal Binding Sites

Testing Sender and Reciever

  • We can test one, then use to test the other, or we can test them in parallel

Testing AgrC Reciever=

  • Use purified AIP
    • Synthesized
    • Sourced
    • S. aureus purified supernatant (requires Pathology department personnel)

Testing AgrA Transcription Factor and P2 promoter

  • Can we get an 'always on' mutant my modifying some residues? (Jim's Idea)

Testing AgrB Exporter

  • Membrane Localization Test
    • Can use membrane fractionation
    • Can use antibody tags with EM and gold

Testing AgrD Prepeptide/AIP

  • Mass Spec Assay
    • Daniel Goodman 11:47, 31 July 2008 (UTC):Speaking to Kathryn Lilley in Proteomics
  • S. aureus Reporter Strain
    • Daniel Goodman 11:47, 31 July 2008 (UTC):We cannot use staph ourselves, checking to see if we can get someone in Pathology department to test for us
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