IGEM:Cambridge/2008/Turing Pattern Formation/Experiments: Difference between revisions
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(New page: ===Finding and Biobricking proper Ribosomal Binding Sites=== *[http://partsregistry.org/Assembly:RBS-CDS_issues Solving Biobrick RBS issues] ===Testing Sender and Reciever=== *We can t...) |
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=Finding and Biobricking proper Ribosomal Binding Sites= | |||
*[http://partsregistry.org/Assembly:RBS-CDS_issues Solving Biobrick RBS issues] | *[http://partsregistry.org/Assembly:RBS-CDS_issues Solving Biobrick RBS issues] | ||
=Testing Sender and Reciever= | |||
*We can test one, then use to test the other, or we can test them in parallel | *We can test one, then use to test the other, or we can test them in parallel | ||
==Testing AgrC Reciever== | |||
*Use purified AIP | *Use purified AIP | ||
**Synthesized | **Synthesized | ||
**Sourced | **Sourced | ||
***Possibly from Tom Muir at Rochester | |||
**S. aureus purified supernatant (requires Pathology department personnel) | **S. aureus purified supernatant (requires Pathology department personnel) | ||
***Guangyong Ji at Catholic University has a protocol for purification | |||
==Testing AgrA Transcription Factor and P2 promoter== | |||
*Can we get an 'always on' mutant my modifying some residues? (Jim's Idea) | *Can we get an 'always on' mutant my modifying some residues? (Jim's Idea) | ||
==Testing AgrB Exporter== | |||
*Membrane Localization Test | *Membrane Localization Test | ||
**Can use membrane fractionation | **Can use membrane fractionation | ||
**Can use antibody tags with EM and gold | **Can use antibody tags with EM and gold | ||
=Testing AgrD Prepeptide/AIP= | |||
*Mass Spec Assay | *Mass Spec Assay | ||
**'''[[User:Daniel Goodman|Daniel Goodman]] 11:47, 31 July 2008 (UTC)''':Speaking to Kathryn Lilley in Proteomics | **'''[[User:Daniel Goodman|Daniel Goodman]] 11:47, 31 July 2008 (UTC)''':Speaking to Kathryn Lilley in Proteomics | ||
*S. aureus Reporter Strain | *S. aureus Reporter Strain | ||
**'''[[User:Daniel Goodman|Daniel Goodman]] 11:47, 31 July 2008 (UTC)''':We cannot use staph ourselves, checking to see if we can get someone in Pathology department to test for us | **'''[[User:Daniel Goodman|Daniel Goodman]] 11:47, 31 July 2008 (UTC)''':We cannot use staph ourselves, checking to see if we can get someone in Pathology department to test for us | ||
Revision as of 07:37, 31 July 2008
Finding and Biobricking proper Ribosomal Binding Sites
Testing Sender and Reciever
- We can test one, then use to test the other, or we can test them in parallel
Testing AgrC Reciever
- Use purified AIP
- Synthesized
- Sourced
- Possibly from Tom Muir at Rochester
- S. aureus purified supernatant (requires Pathology department personnel)
- Guangyong Ji at Catholic University has a protocol for purification
Testing AgrA Transcription Factor and P2 promoter
- Can we get an 'always on' mutant my modifying some residues? (Jim's Idea)
Testing AgrB Exporter
- Membrane Localization Test
- Can use membrane fractionation
- Can use antibody tags with EM and gold
Testing AgrD Prepeptide/AIP
- Mass Spec Assay
- Daniel Goodman 11:47, 31 July 2008 (UTC):Speaking to Kathryn Lilley in Proteomics
- S. aureus Reporter Strain
- Daniel Goodman 11:47, 31 July 2008 (UTC):We cannot use staph ourselves, checking to see if we can get someone in Pathology department to test for us
