IGEM:Cambridge/2008/Turing Pattern Formation: Difference between revisions
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== Overview == | == Overview == | ||
This project seeks to generate [[Wikipedia:Turing Patterns|Turing Patterns]] by creating a [[Wikipedia:Reaction-Diffusion System|Reaction-Diffusion system]] in the [[Wikipedia:gram-positive bacteria|gram-positive bacteria]] [[Wikipedia:Bacillus subtilis|Bacillus subtilis]]. We need to integrate two signalling systems into this bacterium and use an autofeedback mechanism to generate self-organizing patterns from random noise. We plan to incorporate the ''agr'' peptide signalling system from | This project seeks to generate [[Wikipedia:Turing Patterns|Turing Patterns]] by creating a [[Wikipedia:Reaction-Diffusion System|Reaction-Diffusion system]] in the [[Wikipedia:gram-positive bacteria|gram-positive bacteria]] [[Wikipedia:Bacillus subtilis|Bacillus subtilis]]. We need to integrate two signalling systems into this bacterium and use an autofeedback mechanism to generate self-organizing patterns from random noise. We plan to incorporate the ''agr'' peptide signalling system from ''S. aureus'' and the ''lux'' AHL system from ''V. fisheri''. | ||
== Steps/Progress == | == Steps/Progress == | ||
Revision as of 01:09, 21 July 2008
Overview
This project seeks to generate Turing Patterns by creating a Reaction-Diffusion system in the gram-positive bacteria Bacillus subtilis. We need to integrate two signalling systems into this bacterium and use an autofeedback mechanism to generate self-organizing patterns from random noise. We plan to incorporate the agr peptide signalling system from S. aureus and the lux AHL system from V. fisheri.
Steps/Progress
Resources
Notable Papers/Pages
Turing Patterns/Reaction Diffusion Patterns
Short introduction to reaction/diffusion systems and pattern formation
Bacillus subtilis
B. Subtilis Transformation Protcol - Electroporation
Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005) - handy expression system for B. subtilis
- What about feedback/regulation effects of subtilin on the cell
agr system
mutual exclusion between agr systems from different S. aureus strains
lux system
cyclic dipeptides bind competitively and antagonize AHLs in luxR-based signalling..can we exploit?
Quorum Sensing/Cell-Cell Communication
great general overview of QS in both gram-neg and gram-pos bacteria
