IGEM:Cambridge/2008/Turing Pattern Formation: Difference between revisions
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=== agr system === | === agr system === | ||
[http://www.pnas.org/content/96/4/1218.abstract mutual exclusion between agr systems from different S. aureus strains] | |||
=== lux system === | === lux system === | ||
[http://www3.interscience.wiley.com/journal/118790052/abstract cyclic dipeptides bind competitively and antagonize AHLs in luxR-based signalling..can we exploit?] | |||
=== Quorum Sensing/Cell-Cell Communication === | === Quorum Sensing/Cell-Cell Communication === | ||
[http://arjournals.annualreviews.org/doi/full/10.1146/annurev.cellbio.21.012704.131001 great general overview of QS in both gram-neg and gram-pos bacteria] | |||
Revision as of 13:32, 20 July 2008
Overview
This project seeks to generate Turing Patterns by creating a Reaction-Diffusion system in the gram-positive bacteria Bacillus subtilis. We need to integrate two signalling systems into this bacterium and use an autofeedback mechanism to generate self-organizing patterns from random noise. We plan to incorporate the agr" peptide signalling system from "S. aureus and the lux AHL system from V. fisheri.
Steps/Progress
Resources
Notable Papers/Pages
Turing Patterns/Reaction Diffusion Patterns
Short introduction to reaction/diffusion systems and pattern formation
Bacillus subtilis
B. Subtilis Transformation Protcol - Electroporation
Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005) - handy expression system for B. subtilis
agr system
mutual exclusion between agr systems from different S. aureus strains
lux system
cyclic dipeptides bind competitively and antagonize AHLs in luxR-based signalling..can we exploit?
Quorum Sensing/Cell-Cell Communication
great general overview of QS in both gram-neg and gram-pos bacteria
