IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 7: | Line 7: | ||
{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
|-valign="top" | |-valign="top" | ||
|width="20%"|'''Relative Cell Density''' |width="20%"|'''OD600 Reading''' | |width="20%"|'''Relative Cell Density''' | ||
|width="20%"|'''OD600 Reading''' | |||
|-valign="top" | |-valign="top" | ||
|width="20%"|1 | |width="20%"|1 | ||
Revision as of 08:52, 31 July 2008
Method
- A culture of cells was diluted down and the OD600 reading was taken for each.
- Some of the dilutions were plated out at 10-5 and 10-6 fold dilutions, and colonies were counted to determine the total number of cells in the original dilutions.
Results
Relative Cell Density OD600 Reading 1 1.6483 0.8 1.4913 0.6 1.1058 0.4 1.0167 0.2 0.5885 0.1 0.2982 0.08 0.2567 0.06 0.1910 0.04 0.1208 0.02 0.070 0.01 0.0346
