IGEM:Cambridge/2008/Notebook/Voltage/K+ Growth: Difference between revisions
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==Results== | ==Results== | ||
[[Media:MutantGrowthData.xls |Link to raw OD600 Data in Excel]] | |||
[[Image:RelGrowth1mmK.JPG |800px]] | |||
[[Image:RelGrowth50mmK.JPG |800px]] | |||
[[Image:RelGrowth200mmK.JPG |800px]] | |||
Latest revision as of 07:25, 3 September 2008
Method
- For each of the 9 mutants, 3 [K+] of growth medium were examined: 1mM, 50mM & 200mM
Composition of Each Cuvette:
* 100ul cells
* 100ul KCl (10x stocks)
* 800ul LB
----------------------
1ml Final Volume
- The spectrophotometer was zeroed using LB.
- OD readings were taken every 30 minutes for 3 hours.
- In between readings, cultures were stored at room temperature & covered with parafilm.
Results
Link to raw OD600 Data in Excel
