IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(→Method) |
||
| Line 1: | Line 1: | ||
=Method= | =Method= | ||
*OD600 recorded in 1ml cuvette and converted into cell number using [[IGEM:Cambridge/2008/Notebook/Voltage/OD600_Calibration |calibration graph]] | |||
*500ul of this culture was extracted & centrifuged (5 min, low speed.) | |||
*Supernatant removed and discarded (no further analysis due to viscosity problems) | |||
*Pellet washed (resuspend in 1ml SDW and centrifuge 5 min, low speed.) | |||
*Pellet resuspended in 1.5ml SDW, cells then fractured by 3 rounds of freeze-thaw lysis in liquid nitrogen. | |||
*Flame photometry performed on most sensitive setting [[IGEM:Cambridge/2008/Notebook/Voltage/Flame_Photometer_Calibration |(See calibration graph)]] | |||
=Results= | =Results= | ||
Revision as of 07:33, 3 September 2008
Method
- OD600 recorded in 1ml cuvette and converted into cell number using calibration graph
- 500ul of this culture was extracted & centrifuged (5 min, low speed.)
- Supernatant removed and discarded (no further analysis due to viscosity problems)
- Pellet washed (resuspend in 1ml SDW and centrifuge 5 min, low speed.)
- Pellet resuspended in 1.5ml SDW, cells then fractured by 3 rounds of freeze-thaw lysis in liquid nitrogen.
- Flame photometry performed on most sensitive setting (See calibration graph)
