IGEM:Cambridge/2008/Notebook/Voltage
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(Difference between revisions)
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=Aim= | =Aim= | ||
To create a system which responds to ligand binding with a detectable voltage caused by a K<sup>+</sup> flux. | To create a system which responds to ligand binding with a detectable voltage caused by a K<sup>+</sup> flux. | ||
| - | [[IGEM:Cambridge/2008/Notebook/Voltage/Progress | Progress]] | + | [[IGEM:Cambridge/2008/Notebook/Voltage/Progress |<font style="color:#f3ff1f">Progress</font>]] |
=Background= | =Background= | ||
| - | [[Media:Voltage_project.ppt | Presentation]] | + | [[Media:Voltage_project.ppt |<font style="color:#f3ff1f">Presentation</font>]] |
=Experiments= | =Experiments= | ||
| - | [[IGEM:Cambridge/2008/Notebook/Voltage/Mutant Strains | Mutant Strains]] | + | [[IGEM:Cambridge/2008/Notebook/Voltage/Mutant Strains |<font style="color:#f3ff1f"> Mutant Strains</font> ]] |
| - | [[IGEM:Cambridge/2008/Notebook/Voltage/Flame Photometer Calibration|Flame Photometer Calibration]] | + | [[IGEM:Cambridge/2008/Notebook/Voltage/Flame Photometer Calibration|<font style="color:#f3ff1f">Flame Photometer Calibration</font>]] |
| - | [[IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations|K+ Concentrations]] | + | [[IGEM:Cambridge/2008/Notebook/Voltage/K+ Concentrations|<font style="color:#f3ff1f">K+ Concentrations</font>]] |
| - | [[IGEM:Cambridge/2008/Notebook/Voltage/BioBrick Manipulation|BioBrick Manipulation]] | + | [[IGEM:Cambridge/2008/Notebook/Voltage/BioBrick Manipulation|<font style="color:#f3ff1f">BioBrick Manipulation</font>]] |
| - | [[IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration|OD600 Calibration]] | + | [[IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration|<font style="color:#f3ff1f">OD600 Calibration </font> ]] |
=Next Steps= | =Next Steps= | ||
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=Useful Links= | =Useful Links= | ||
| - | [http://expasy.org/tools/ Protein prediction tools] | + | [http://expasy.org/tools/ <font style="color:#f3ff1f">Protein prediction tools</font>] |
| - | [http://www.uniprot.org/ Uniprot database] | + | [http://www.uniprot.org/ <font style="color:#f3ff1f">Uniprot database</font>] |
=Literature= | =Literature= | ||
| - | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1214631 Kdp operon diagram] | + | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1214631|<font style="color:#f3ff1f"> Kdp operon diagram</font>] |
| - | [http://www.jbc.org/cgi/content/abstract/276/13/9590 Kdp plasmid] | + | [http://www.jbc.org/cgi/content/abstract/276/13/9590|<font style="color:#f3ff1f"> Kdp plasmid</font>] |
| - | [http://www.springerlink.com/content/6042632827845551/ | + | [http://www.springerlink.com/content/6042632827845551/ <font style="color:#f3ff1f"> The Kdp-ATPase system and its regulation</font>] |
| - | Potential Chassis: [http://cgsc.biology.yale.edu/Strain.php?ID=107402 Strain JW1242-1] | + | Potential Chassis: [http://cgsc.biology.yale.edu/Strain.php?ID=107402 |<font style="color:#f3ff1f">Strain JW1242-1</font>] |
| - | [http://cgsc.biology.yale.edu/Strain.php?ID=107065 Strain JW0710-1] | + | [http://cgsc.biology.yale.edu/Strain.php?ID=107065 <font style="color:#f3ff1f">Strain JW0710-1</font>] |
| - | [http://www.ncbi.nlm.nih.gov/pubmed/4942756 Kdp mutant - paper from 1971] | + | [http://www.ncbi.nlm.nih.gov/pubmed/4942756 <font style="color:#f3ff1f">Kdp mutant - paper from 1971</font>] |
| - | [http://www.shigen.nig.ac.jp/WGR/link/link_E.coli_e.html Worldwide E.coli Databases] | + | [http://www.shigen.nig.ac.jp/WGR/link/link_E.coli_e.html <font style="color:#f3ff1f"> Worldwide E.coli Databases</font>] |
| - | [http://jb.asm.org/cgi/content/abstract/188/5/1950 Characterisation of kdpD - 2005] | + | [http://jb.asm.org/cgi/content/abstract/188/5/1950 <font style="color:#f3ff1f">Characterisation of kdpD - 2005</font>] |
| - | [http://jb.asm.org/cgi/content/full/180/19/5102 Investigations on Kdp Operon exp. & flux] | + | [http://jb.asm.org/cgi/content/full/180/19/5102 <font style="color:#f3ff1f">Investigations on Kdp Operon exp. & flux</font>] |
| - | [http://dx.doi.org/10.1006/jmbi.2001.4884 Very interesting 2001 paper concerning Glutamate Channels] | + | [http://dx.doi.org/10.1006/jmbi.2001.4884 <font style="color:#f3ff1f"> Very interesting 2001 paper concerning Glutamate Channels</font>] |
| - | [http://www.nature.com/nature/journal/v402/n6763/full/402817a0.html 1999 paper on functional characterization of prokaryote Glu Channels] | + | [http://www.nature.com/nature/journal/v402/n6763/full/402817a0.html <font style="color:#f3ff1f">1999 paper on functional characterization of prokaryote Glu Channels</font>] |
| - | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=BA000022 Sequenced Synechocystis PCC 6803 genome] | + | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=BA000022 <font style="color:#f3ff1f"> Sequenced Synechocystis PCC 6803 genome</font>] |
| - | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=47118304&from=1401809&to=1403002&view=gbwithparts Glutamate-gated K+ channel GluR0] | + | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=47118304&from=1401809&to=1403002&view=gbwithparts <font style="color:#f3ff1f"> Glutamate-gated K+ channel GluR0</font>] |
| - | [http://redpoll.pharmacy.ualberta.ca/CCDB/cgi-bin/STAT_NEW.cgi Link to E.coli statistics page (CCDB Database)] | + | [http://redpoll.pharmacy.ualberta.ca/CCDB/cgi-bin/STAT_NEW.cgi|<font style="color:#f3ff1f"> Link to E.coli statistics page (CCDB Database)</font>] |
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Revision as of 05:42, 26 August 2008
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