IGEM:Cambridge/2008/Notebook/Voltage

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 7: Line 7:
{|{{table}} width="810"
{|{{table}} width="810"
|-
|-
-
|style="background-color: #cdde95;" align="center"|
+
|style="background-color: #c9ffe5;" align="center"|
<!-- ## START calendar column -->
<!-- ## START calendar column -->
<LNCalendar></LNCalendar>
<LNCalendar></LNCalendar>
<!-- ## END calendar column -->
<!-- ## END calendar column -->
-
|align="center" style="background-color: #e5edc8;" |
+
|align="center" style="background-color: #000000;" |
<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
[[Image:Voltage_logo.jpg‎ |300px]] <sitesearch>title=Search this project</sitesearch>
[[Image:Voltage_logo.jpg‎ |300px]] <sitesearch>title=Search this project</sitesearch>

Revision as of 17:29, 19 August 2008



Search this project

Customize your entry pages

Aim

To create a system which responds to ligand binding with a detectable voltage caused by a K+ flux.

Progress

Background

Presentation

Experiments

Mutant Strains

Flame Photometer Calibration

K+ Concentrations

BioBrick Manipulation

OD600 Calibration

Next Steps

1. Ligate OsmY, RBS, and KDP in sequence into a vector. Possible difficulty due to the size of the KDP gene.

2. Determine the correct mutant to use:

  • Determine transformation efficiency of mutants.
  • Plot growth curves of mutants in normal/varying K+ conditions.
  • Estimate K+ uptake in varying K+ conditions.

3. Acquire ligand gated ion channel (GluR0)

Useful Links

Protein prediction tools

Uniprot database

Literature

Kdp operon diagram

Kdp plasmid

The Kdp-ATPase system and its regulation

Potential Chassis: Strain JW1242-1 Strain JW0710-1

Kdp mutant - paper from 1971

Worldwide E.coli Databases

Characterisation of kdpD - 2005

Investigations on Kdp Operon exp. & flux

Very interesting 2001 paper concerning Glutamate Channels

1999 paper on functional characterization of prokaryote Glu Channels

Sequenced Synechocystis PCC 6803 genome

Glutamate-gated K+ channel GluR0

Link to E.coli statistics page (CCDB Database)

Recent changes



Personal tools