IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/15: Difference between revisions
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- Nanodrop | - Nanodrop | ||
{|class="wikitable" style="text-align:center" border="1" | |||
|- | |||
! product !! concentration (ng/μL) !! 260/280 | |||
|- | |||
| RBS W2 || 90.3 || 1.70 | |||
|- | |||
| RBS W4 || 124.0 || 1.80 | |||
|} | |||
- Single digest with EcoRI, XbaI, SpeI and PstI | |||
- Gel | |||
*Lane1 : HyperladderI | |||
*Lane2 : RBS S6 cut with EcoRI | |||
*Lane3 : RBS S6 cut with SpeI | |||
*Lane4 : RBS S6 cut with PstI | |||
*Lane5 : RBS S6 cut with XbaI | |||
*Lane6 : RBS S6 uncut | |||
*Lane7 : RBS W6 cut with EcoRI | |||
*Lane8 : RBS W6 cut with SpeI | |||
*Lane9 : RBS W6 cut with PstI | |||
*Lane10 : RBS W6 cut with XbaI | |||
*Lane11 : RBS W6 uncut | |||
*Lane12 : supercoiled ladder | |||
*Result | |||
Revision as of 03:20, 16 September 2008
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | ||||||||||
Check big stock of RBS S and W
- Plasmid miniprep RBS S6 and RBS W6 (from 200mL flask) - Nanodrop
- Single digest with EcoRI, XbaI, SpeI and PstI - Gel
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